摘要
Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ大鼠经皮下接种Ｗａｌｋｅｒ２５６癌肉瘤细胞后以１％精氨酸溶液代替饮用水饲喂大鼠分别进行肿瘤、肝脏、肌肉和整体的蛋白质代谢分析。精氨酸治疗后肿瘤体积和重量显著减少，肿瘤蛋白质合成率、分段生长率均显著减少，而肿瘤蛋白质分段降解率则无明显改变。精氨酸也影响宿主蛋白质代谢。肝脏蛋白质合成率以及整体蛋白质分段合成率也明显减少，但肌肉蛋白质合成则无明显改变。结果表明，补充精氨酸可减少肿瘤蛋白质的合成和抑制肿瘤的生长，同时也使宿主蛋白质代谢发生改变。
Effects of supplementation of arginine on protein metabolism of malignant tumor and the host were investigated in cancer-bearing rats. Sprague-Dawley rats were subcutaneonsly inoculated with Walker 256 carcinos arcoma cells and received 1% arginine solution as drinking water. The rats were injected intraperitoneally with floodIng dose of leucine and14 C-leucine on day 14 after tumor implantation and Specimens of tumor,liver,muscle and whole body were prepared for analysis of protein metabolism,Arginine drinking resulted in significant decrease of tumor protein synthesis(0.45±0.15g/d d versus control 1.14±0.20g/d,P<0.01),tumor fractional growth rate(26.4±4.%/d versus control 35.8±2.3%/d,P<0.05)in arginine group but no significant change of tumor protein fractional degradation rate.Significant decrease was also found in arginine group for liver protein fractional synthesis rate(100.7±18.4%/d versus control 166.6±19.0%/d,P<0.05)and protein synthesis(1.85±0.35/d versus control 3.36±0.32 g/d P<0.01)as well as whole body protein fractional synthesis rate(39.3±3.8%/d versus control 50.2±2.2%/d,P< 0.05),These results further proved that supplemented arginine decreased tumor protein synthesis and tumor growth,as well as affected host protein synthesis.Thus supplementation of arginine may benefit to anti-tumor therapy.
出处
《上海医科大学学报》
CSCD
1995年第4期250-253,共4页
Journal of Hehai University(Medical Science)
基金
美国国立卫生研究院科学基金
关键词
精氨
蛋白质
肿瘤
宿主
arginine
protein metabolism
tumor
host
