摘要
目的观察雷公藤甲素对胶原诱导的关节炎大鼠关节局部热休克蛋白(HSPs)和主要组织相容性复合体类分子(MHC-)表达的影响。方法建立胶原诱导的关节炎大鼠模型,雷公藤甲素按40μg/kg im给药,运用逆转录聚合酶链反应(RT-PCR)和免疫酶组织化学染色的方法,观察大鼠关节滑膜细胞和软骨细胞HSP60、HSP70和MHC-类分子的表达情况。结果与正常对照组相比,模型组大鼠关节滑膜细胞及软骨细胞HSP60、HSP70和MHC-类分子的表达均显著增高(P<0.05),与模型组相比,雷公藤甲素可以下调关节炎大鼠关节局部HSP60、HSP70和MHC-类分子的表达(P<0.05)。结论降低关节炎大鼠关节局部软骨细胞和滑膜细胞异常表达的HSPs与MHC-类分子的表达,可能是雷公藤甲素治疗类风湿关节炎(RA)的作用机制之一。
Objective To investigate the effects of triptolide on the expression of heat shock proteins(HSPs) and major histocompatibility-(MHC-) molecule in synoviocytes and chondrocytes of collagen-induced arthritis rats.Methods The experimental Wistar rat model of collagen-induced arthritis was es-tablished.The rats in triptolide group were administered with triptolide at 40μg/kg by im.The expres-sion of HSP60,HSP70,and MHC-molecule in synoviocytes and chondrocytes was detected by RT-PCRand immunohistochemical staining.Results To compare with normal group,the expression of HSPs andMHC-molecule increased remarkably in synoviocytes and chondrocytes of model rats(P<0.05).Trip-tolide could reduce the expression of these molecules in inflammatory joints rats(P<0.05).Conclusion Triptolide may play its curative role via down-regulating the exprssion of HSPs and MHC-molecule insynoviocytes and chondrocytes of rheumatoid arthritis(RA).
出处
《中草药》
CAS
CSCD
北大核心
2005年第8期1193-1196,共4页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30070961)
