摘要
贮脂细胞是目前肝纤维化研究的热点。本文参考Ｆｒｉｅｄｍａｎ等的不连续密度梯度离心法并作改良，建立了一种经济、简便、可靠的分离大鼠肝贮脂细胞、Ｋｕｆｆｅｒ细胞的方法。贮脂细胞得率约１０７／大鼠，纯度为９０％以上，Ｋｕｐｆｆｅｒ细胞纯度约６０％，其培养上清基本适合进一步研究用。本方法的建立，为进一步研究肝贮脂细胞与肝纤维化的关系，尤其是细胞水平及分子生物学水平的研究奠定了基础。
at-storing cells(FSC or Ito cells) have become
the focus in the study of liver fibrogensisat present. A modified simple and efficient density
gradient centrifugation method wasestablished in this paper. The yield rate was about l07 per
rat. The purity of FSC and Kupffercells (KC) was around 90%and 60, respectively. The survival
rate of the cells was about90%. This modified method of preparing FSC and KC may provide the
basis for the study ofliver fibrosis at the cellular and molecular level.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1995年第3期142-145,共4页
Chinese Journal of Digestion
