摘要
通过丛生芽直接增殖和愈伤组织再分化两条途径建立紫茎泽兰的无性系,可为进一步研究紫茎泽兰入侵的分子生态机制提供植物材料。结果表明:MS+BA2.0mg·L-1+NAA0.05mg·L-1为丛生芽增殖的最适培养基,MS+BA0.1mg·L-1+NAA0.1mg·L-1和MS+BA2.0mg·L-1+NAA0.2mg·L-1分别适于叶片愈伤组织的诱导和分化,而1/2MS+IAA0.1mg·L-1对生根最有利。
Direct shoot multiplification and callus differentiation by tissue culture were adopted to develop Eupatroium adenophorum clones,which could be used as the material for further studying the infesting mechanism in molecular ecology.The results showed that the optimal medium for its direct shoot multiplification was MS+BA2.0 mg·L^(-1)+NAA 0.05 mg·L^(-1) and the optimal mediums for the induction and differentiation of its leaf tissue callus were MS+BA 0.1 mg·L^(-1)+NAA 0.1 mg·L^(-1) and MS+BA 2.0 mg·L^(-1)+NAA 0.2 mg·L^(-1),respectively;moreover,the best medium for rooting was 1/2 MS+IAA (0.1) mg·L^(-1).
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第7期1458-1462,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家973研究项目(2002CB111402-3-6)
国家自然科学基金(30170619)
关键词
紫茎泽兰
丛生芽增殖
愈伤组织
再分化
Eupatroium adenophorum
bud multiplification
callus
differentiation
