摘要
实时PCR是在常规PCR基础上运用荧光共振能量转移现象,加入荧光标记探针,巧妙地把核酸扩增、杂交、光谱分析和实时检测技术结合在一起的一项新技术,具有快速、灵敏、特异性强、定量准确等特点,广泛应用于医学、检验检疫、军事、农业、基础研究等领域。着重就实时PCR技术的特性及在植物上的应用进行了讨论,并与目前常用的相关技术进行了比较。
Real-time PCR differs from classical PCR in the measurement of the amplified PCR product at each cycle throughout the PCR reaction. It allows the detection of a given nucleic acid target in a rapid, specific and very sensitive way, as well as it affords the absolute quantification of the initial target. Up to date, it is widely used in the domain of medicine, inspection and quarantine, military affairs, agriculture and foundation study. The technical features of quantitative real-time PCR that are especially relevant to plant research were described, and its present and future application were summarized. The technical qualities of real-time PCR, compared with the other currently used techniques, are also discussed.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2005年第5期15-21,共7页
China Biotechnology
基金
国家重点基础研究发展计划项目 (G19980 10 2 0 4)
浙江省自然科学基金项目 (M3 0 3 3 87)
