摘要
作者观察了全反式维甲酸（ａｌｌｔｒａｎｓＲｅｔｉｎｏｉｃＡｃｉｄ，ＲＡ）对一个克隆化高转移人肺癌细胞（ＰＧＣＬ３）粘附性和运动性的影响。结果显示，经ＲＡ处理的ＰＧＣＬ３细胞，以层粘连蛋白为粘附基质的粘附百分率以及Ｂｏｙｄｅｎ１小室的穿膜运动能力均受到抑制，其抑制作用随ＲＡ浓度增高而更加明显步进行细胞免疫组化和ＤＮＡ－ＲＮＡ斑点杂交实验，结果表明，以１０μｍｏｌ／ＬＲＡ处理ＰＧＣＬ３细胞，可以降低６７－ＫＤ层粘连蛋白受体（ＬＮ－Ｒ）基因的表达，并且在１０μｍｏｌ／ＬＲＡ处理的ＰＧＣＬ３细胞中，自分泌运动因子受体（ＡＭＦ－Ｒ）的表达亦有明显下降。提示上述二种基因表达的下降是ＲＡ抑制ＰＧＣＬ３细胞粘附和运动能力的重要基础。
Abstract Effects of all-trans Retinoic
Acid(RA)on the adhesion and motility of metastatic human lungcancer cell subline (PGCL3)were
observed in vitro. The results showed that treatment of PGCL3with RA for 5 days decreased the
adhesion of cells to laminin substrate and the migrative abilitythrough the polycarbonate filter
of Boyden chamber,and those inhibitory effects became more obviouswith the increase of RA
concentration. Further investigation by DNA-RNA dot blot hybridizationand
immunohistochemistry denoted that RA-treated PGCL3 cells expressed lower level of
67-KDLN-R compared to untreated cells. The data from DNA-RNA dot blot hybridization also
showedthat RA could reduce the expression of AMF-R significantly. These results raise the
possibility thatthe previously reported suppression by RA of PGCL3 invasion and metastasis
may be related to suppresion of cell adhesion and motility resulting from the decreased
expression of the LN-R andAMF-R respectively.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1994年第5期323-326,共4页
Chinese Journal of Oncology
基金
国家"八.五"攻关课题
