摘要
目的 探讨雌激素对大鼠视网膜缺血再灌注所致视网膜损伤的保护作用。 方法 60只去势雌性SD大鼠随机分为2组,行前房灌注,建立视网膜缺血再灌注(RIR)模型。实验组在升高眼压前2h按10 0 μg/kg的剂量皮下注射17β雌二醇。对照组大鼠皮下注射等量生理盐水。分别在灌注前,再灌注后12、2 4、48、72h对视网膜进行常规HE染色切片,观察细胞丢失情况及测量视网膜内层厚度。采用末端脱氧核酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法(TUNEL法)检测视网膜组织中凋亡细胞的表达。 结果 实验组再灌注后2 4、48h的凋亡细胞数目明显低于对照组(P <0 .0 5 ) ,光学显微镜下计数视网膜神经节细胞数较对照组多(P <0 .0 5 )。 结论 雌激素对缺血再灌注所导致的视网膜损伤具有保护作用。
Objective To evaluate the protective effect of estrogen on survival of retinal ganglion cells (RGCs) after transient retinal ischemia-reperfusion (RIR) in rats. Methods RIR was induced in 60 ovariectomized adult rats (OVX) by increasing intraocular pressure via an intracameral catheter. All of the rats were divided into two groups randomly: in experimental group, the rats underwent a subcutaneous injection with 17β-estrodiol(100 μg/kg) 2 hours before retinal ischemia; and in the control group, saline water was injected correspondingly. The number of RGCs and the thickness of the inner retinal layers were mesured by HE staining method before and 12, 24, 48, and 72 hours after reperfusion. TdT-mediated biotin-dUTP nick end labelling (TUNEL) staining technique was used to examine the apoptosis of RGCs. Results Twenty-four and 48 hours after reperfusion, the number of apoptotic cells in experimental group was obvious lower than that in the control group( P <0.05), and the number of RGCs in experimental group was higher than that in the control group( P <0.05). Conclusion Estrogen can protect retinal neurons from transient RIR in ovariectomized rats.
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
2005年第3期177-179,共3页
Chinese Journal of Ocular Fundus Diseases
