摘要
目的 研究正常人睫状体是否有单纯疱疹病毒Ⅰ型潜伏。方法 采用健康志愿者捐献的新鲜眼球15个 1/3眼球组织进行免疫组化染色,排除HSV -1急性感染。取出剩余的睫状体,1/3睫状体组织进行离心,取上清液与Vero细 胞共培养,另外2/3睫状体组织提取DNA作PCR和凝胶电泳检测HSV -1的DNA和LAT。结果 15个标本免疫组化染色 皆阴性,Vero细胞与睫状体组织上清液共培养结果为阴性,未观察到细胞病理学改变,PCR检测的15个标本中4、5、7号标 本的HSV- 1DNA阳性,HSV -1LAT阴性,其他12个标本PCR检测HSV -1DNA阴性,HSV --1LAT阴性。结论 正常人睫状体 存在HSV 1DNA。
Objective To investigate whether there is latency of herpes simplex virus 1 (HSV-1) in the ciliary body of normal human eyes. Methods Parts (1/3) of the 15 fresh eyeballs from normal healthy donors were used for immunohistochemical staining to exclude from acute infection of HSV-1. After the residual ciliary bodies were taken out, 1/3 was centrifugated for co-culture with Vero cells, and DNA was extracted from the other 2/3 for PCR and gel electrophoresis for the detection of HSV-1 DNA and latency associated transcript (LAT). Results All the 15 samples were negative of immunohistochemical staining and the results of co-culture of Vero cells with the supernatant containing the ciliary body tissues were also negative. No pathological changes in the cells were observed. In the 15 samples, no. 4, 5, 7 samples were positive of HSV-1 DNA but negative of HSV-1 LAT, whereas the other 12 samples were negative of HSV-1 DNA and HSV-1 LAT. Conclusion HSV-1 DNA exists in the normal human cilicary body.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第5期429-431,共3页
Journal of Third Military Medical University
关键词
HSV-1
潜伏
睫状体
herpes simplex virus type 1
latency
ciliary body
