摘要
通过对32例人乳腺良、恶性肿瘤的衰减全反射红外光谱的对比研究发现,它们之间存在明显而规律的光谱差异,反映了乳腺良、恶性病变组织中,蛋白质、核酸和脂类等生物大分子的构型与构象以及相对含量上的差别研究表明傅里叶变换红外光谱可以从分子水平上揭示良、恶性肿瘤的分子结构信息。
Attenuated total reflection (ATR) FTIR spectra of benign and malignant breast tumor tissues from 32 female patients have been determined and investigated. The spectral differences and changes (shape, peak position and relative intensities) have been observed between these two types of samples. In comparison with the benign tumor tissues, the mainly characteristic changes of malignant breast tissues involve: 1) The secondary structure of proteins in malignant tumor tissues is composed mainly of a-helix and random coil, in which a-helix structure is dominant. 2) The absorption band near 1160 cm-1 due to the C-O(H) stretching modes of serine, threonine, tyrosine groups evidently shifted to a higher wavenumber, suggesting that the intermolecular hydrogen bonds were nearly broken, and the collagen absorption peaks at 1205 cm-1, 1278 cm-1 and 1338 cm-1 became lower or disappeared, showing its relative contents decreased. 3) The bands from the symmetric and asymmetric stretching modes of the PO2- of the phosphodiester groups in nucleic acid shifted to 1083 cm-1 and 1242 cm-1, respectively, and became stronger. All these indicated that the relative content of nucleic acid increased greatly. 4) The A2852/A2870 and A2923/A2958 absorbance ratio were significantly higher for malignant tumor tissues than for benign breast tumor tissues. This suggested that there were changes in the conformational structure of the methylene chains of membrane lipids. These results showed that ATR-FTIR spectroscopy was able to become a powerful tool for the biochemical study and the vivo diagnosis of human breast cancers.
出处
《光子学报》
EI
CAS
CSCD
北大核心
2005年第3期390-394,共5页
Acta Photonica Sinica
基金
国家教育部"振兴"计划基金资助项目
关键词
衰减全反射
傅里叶变换红外光谱
乳腺肿瘤组织
蛋白质二级结构
氢键
Attenuated Total Reflection (ATR)
Fourier transformation infrared spectroscopy
Mlignant breast tumor tissue
Secondary structure
Hydrogen bond
