摘要
研究以名贵月季品种“落霞”、“墨红”、“和平”、“金背大红”等为试材，以ＭＳ基本培养基附加不同浓度的６－ＢＡ、ＮＡＡ，以诱导腋芽增殖为微繁途径。结果表明：无菌外植体的建立培养基为ＭＳ＋６－ＢＡＯ．５－２＋ＮＡＡＯ．０５－０．１＋Ｖｃ１００；芽增殖培养基为ＭＳ十ＢＡ１＋ＮＡＡ０．０５，在此培养基上繁殖系数为５．５；生根培养基为ＭＳ＋ＩＡＡ１＋ＩＢＡ１＋ＮＡＡ０．１－肌醇，生根率在９５％左右。生根苗移栽成活率在８５％左右。同时对繁殖工艺流程、成本、商品苗产率等问题进行了讨论，给出在繁殖系数为Ｆ、生根数为ｂ时，有效繁殖系数ｆ＝Ｆ—ｂ，试管苗实际增殖倍数Ｎｎ＝（Ｆ—ｂ）（ｎ－１）·Ｆ、生根苗数Ｒ１－ｎ＝ｆ－１；当生根率为ｒ１，移栽成活率为ｒ２，则商品苗产率为Ｃ＝ｒ１·ｒ２·Ｒｎ＝ｒ１·ｒ２·ｂ（ｆ－１）／（ｆ－１）
The micropropagation of rose cultivars, Peace, Lady Sylva, Condesa de Saatago, Crimson Glory, in basic MS with different concentration of 6-BA and NAA, as a way of micropropagation inducing axillary bud proliferation, was reported in this paper. The medium for establishing no bacteria explants is basic MS with 6-BA 0.5-2mg/L, NAAO.05-0.1mg/L,Vc100mg/L; for proliferating buds is MS with 6BAlmg/L, NAAO. 05mg/L and on this medium the propagation coefficient of buds is 5.5; for rooting is 1/2 MS with IAAl, IBAl, NAAO. 1, eliminated inositol. The rate of rooting is 95%, and the rate of transplanting seedling is 85%.In this paper, the problem of technological process of micropropagation, production cost, rate of commercial seedling, were discussed. AS the propagation coefficient is F, the number of rooting iS b, then the effetive propagation coefficient is f=F-b, the practice proliferation multiple of tube Seedling is Nn=F. (F-b)(n-1). The number of rooting Seedling is R1-n=b(fn-1)/f-1. As the rooting rate is rl, transplanting rate is r2, then, the rate of cornmercial seedling is C=r1. r2. Rn= r1.r2 .b(fn-1)/f-1.
出处
《生物技术》
CAS
CSCD
1994年第4期19-23,共5页
Biotechnology
基金
甘肃教委基金
关键词
月季
微繁
繁殖
培养基
扦插
Rose chenensis
Micropropagntion
Technology
