摘要
本文研究了枯草芽孢杆菌Ｂ＿３菌株经种子处理后在小麦根系及茎基部的定殖能力和影响其定殖的可能因子。结果表明，Ｂ＿３在田间自然土壤中定殖小麦根系及茎基部的时间分别为６０天和４２天以上。培养不同时间的Ｂ＿３菌悬液处理种子表明，播后２２天内，培养２４小时的菌在小麦上的定殖量高于培养４８和７２小时。用ＮＢ培养的Ｂ＿３菌悬液经离心弃上清，并用新鲜ＮＢ液悬浮菌体后处理种子，在播后１９天，Ｂ＿３定殖量比不处理菌悬液的定殖量增加。Ｂ＿３与化学杀菌剂混合处理种子，以及土壤经灭菌处理均可增加Ｂ＿３在小麦上的定殖量。
olonizing activities of B_3 (Bacillus subtilis ) on wheat root and
stem-base and itseffecting-factors were studiedin bour laboratory.It was found tkat in natural soils,
B3can colonize and survivc over 60 and 42 days on wheat root and stem-base, respectively.B3
grew better when seeds are coated with 24 hour-old culture of B3 than those with 48or 72 hour-old
cultures.Addition of NB to B3 inoculum,mixed treatment of B3 withfungicides or soil-sterile out
increased B3 population on wheat roots.
基金
85-10-02-04课题
关键词
枯草芽孢杆菌
定殖
小麦
Bacillus subtilis colonization wheat
