摘要
本实验采用Ｎｏｒｔｈｅｒｎ印迹杂文方法研究了甲基丙烯酸环氧丙酯（ＧＭＡ）对金仓鼠胚胎（ＳＨＥ）细胞原癌基因Ｃ─ｍｙｃ和重要功能基因谷胱甘肽Ｓ─转移酶ｐ（ＧＳＴ─ｐ）表达的影响．结果表明ＧＭＡ促进了这些基因的表达，其中ＧＳＴ─ｐ基因在作用２ｈ表达即见增高，５ｈ后达到高水平，直至１２ｈ仍维持这一高水平；ｃ─ｍｙｃ基因在１２ｈ后出现高水平．本文讨论了ＧＭＡ诱导上述基因表达的改变与细胞转化之间的可能关系．
To explore preliminaryly the mechanism of golden Syrian hamster
embryo(SHE)cells transformation induced by glycidyl methacrylate(GMA),the
transeriptionalactivities of some singificant genes such as protooncogene myc(c─myc)and
glutathioneS─transferase─p(GST─p)in the SHE cells treated with GMA were examined by
usingNorthern blot hybridization with corelative labelled probes。It was observed that the
ex-pression level of GST─p m RNA began to increas at 2 hours,and reached to a high
steadylevel from 5 hours to 12 hours after the SHE cells were trcated with GMA. The
expressionof protooncogene myc reached a peak at l2 hours after the SHE cells treated with
GMA.The elevation of the transcription of the genes showed that the regulation of expression
ofthe cell genes might be effected by the interference of GMA. We concluded that the activation
of expression of c─myc and GST─p gene is important and an initiation step dur- ing the
transformation of SHE cells by GMA was discussed.
出处
《癌变．畸变．突变》
CAS
CSCD
1994年第1期6-10,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金
关键词
甲基丙烯酸
环氧丙酯
原癌基因
glycidyl methacrylate
protooncogene myc
glutathione S─transferase─p
