摘要
为研究载脂蛋白E(ApoE)基因多态性与中国东北汉族2型糖尿病合并脑梗死的关系,采用聚合酶链反应 限制性片段长度多态性(PCR RFLP)技术,检测了208例个体的ApoE基因多态性,其中对照(CON)组69例,2型糖尿病无大血管病变(T2DM)组67例和2型糖尿病合并脑梗死(T2DMCI)组72例;同时测定了其中70例T2DMCI患者的血脂含量。CON组等位基因频率为:ε29.6%、ε382.4%、ε48.1%,基因型频率为:ε2ε313.2%、ε3ε367 6%、ε3ε416.2%;T2DM组等位基因频率为:ε210.5%、ε384.3%、ε45.2%,基因型频率为:ε2ε319.4%、ε3ε370.1%、ε3ε49%;T2DMCI组等位基因频率为:ε211.8%、ε384.7%、ε43.5%,基因型频率为:ε2ε315.2%、ε3ε375%、ε3ε44.2%。3组间等位基因和基因型频率的差异经检验无统计学意义。T2DMCI患者中各基因型之间的血浆总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL C)和低密度脂蛋白胆固醇(LDL C)水平亦无显著性差异。在中国东北汉族人群中,未发现ApoE基因多态性与T2DMCI之间存在关联,亦未发现ApoE基因多态性与T2DMCI患者的TC、TG、HDL C和LDL C水平之间存在关联。
In order to explore the association of apolipoprotein E (ApoE) gene polymorphism with cerebral infarction in type 2 diabetic patients of Han nationality in Northeast China, the genotypes of ApoE gene were analyzed by polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) in the 208 cases, including 69 cases in control (CON) group, 67 in type 2 diabetes mellitus (T2DM) group and 72 in type 2 diabetes mellitus with cerebral infarction (T2DMCI) group. Plasma lipid content in T2DMCI group was also detected in 70 cases. The distribution of genotypes in ApoE gene,ε_2ε_3,ε_3ε_3 as well as ε_3ε_4 was no significant difference in three groups (ε_2ε_3 : 13.2%,ε_3ε_3 : 67.6%,ε_3ε_4 : 16.2%in CON group;ε_2ε_3 : 19.4% ε_3ε_3: 70.1%ε_3ε_4 : 9% in T2DM group;ε_2ε_3 : 15.2%,ε_3ε_3 : 75%,ε_3ε_4 : 4.2%in T2DMCI group).The allele frequencies ofε_2,ε_3 and ε_4 were not significantly different in the three groups, either (ε_2 : 9.6%,ε_3 : 82.4%,ε_4 : 8.1% in CON group; ε_2 :10.5%,ε_3 :84.3%,ε_4 : 5.2% in T2DM group; ε_2 :11.8%,ε_3 :84.7%,ε_4 : 3.5% in T2DMCI group). The levels of total cholesterol (TC), tryglyceride (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were not significantly different among the different genotypes in T2DMCI group. The study confirmed that the polymorphisms of ApoE gene were neither associated with the T2DMCI, nor with the levels of plasma lipid in T2DMCI.
出处
《遗传》
CAS
CSCD
北大核心
2005年第1期35-38,共4页
Hereditas（Beijing)
基金
黑龙江省自然科学基金资助(D-0357)
黑龙江省卫生厅科研基金资助(2003-149)~~
关键词
载脂蛋白E
基因多态性
2型糖尿病
脑梗死
apolipoprotein E
gene polymorphism
type 2 diabetes mellitus
cerebral infarction
