摘要
目的 :探讨内皮型一氧化氮合成酶 (eNOS)、细胞增殖标记物MIB 1在幕上星形细胞瘤的表达及相互关系 ,明确它们在星形细胞瘤病理机制中的作用 .方法 :采用免疫组织化学技术 ,检测 5例正常脑组织及 4 7例幕上星形细胞瘤手术切除标本中的eNOS和MIB 1表达 ,观察表达结果与肿瘤生物学特性之间的关系 .结果 :eNOS在正常脑组织无表达 .在低度恶性星形细胞瘤 (Ⅰ ,Ⅱ )中 ,eNOS轻度染色 15例 ,中度染色11例 .在恶性星形细胞瘤 (Ⅲ )中 ,轻度染色 1例 ,中度染色 6例 ,高度染色 2例 .高度恶性星形细胞瘤 (Ⅳ )中 ,中度染色 2例 ,高度染色 10例 .MIB 1阳性细胞计数在低度恶性星形细胞瘤为 2 1,恶性星形细胞瘤为 8 9,高度恶性星形细胞瘤为2 4 5 ,正常脑组织无表达 .在eNOS表达轻度、中度、高度 3级别中 ,MIB 1阳性细胞记数分别为 2 2 3,6 2 9和 2 0 4 0 .结论 :eNOS在星形细胞瘤血管内皮细胞的表达与组织病理学分级及肿瘤细胞增殖潜能密切相关 .
AIM: To study the relationship and the expression of endothelial nitric oxide synthase (eNOS) and cell proliferating marker MIB-1 in superatentorial astrocytic tumors, and to describe their functions in the pathological mechanism of human superatentorial astrocytic tumors. METHODS: Tissue samples were obtained from 26 patients with low-grade astrocytomas (gradeⅠand Ⅱ), 9 with anaplastic astrocytomas(grade Ⅲ), and 12 with high-grade astrocytomas (grade Ⅳor glioblastomas). Normal brain tissue samples were obtained from 5 patients with other brain diseases. Immunohistochemical staining was performed using the avidin-biotin complex method to examine the expression of eNOS and MIB-1. The levels of eNOS expression in endothelial cells were evaluated as slight (+), moderate (), and intense (). The cell proliferating marker MIB-1 was assessed as MIB-1 cell count for tumor cells. RESULTS: The expression of eNOS was negative in all the specimens of normal brain tissue, slight in 15 and moderate in 11 specimens of low-grade astrocytoma, slight in 1, moderate in 6 and intense in 2 of anaplastic astrocytoma, and moderate in 2 and intense in 10 specimens of glioblastoma. The MIB-1 cell count was 2.1 for low--grade astrocytomas, 8.9 for anaplastic astrocytomas, and 24.5 for glioblastomas. The MIB-1 cell count for slight, moderate and intense eNOS expression was 2.23, 6.29, and 20.40, respectively. CONCLUSION: The expression of eNOS and MIB-1 are significantly correlated with the histopathologicol grade or malignancy in human astrocytic tumors. eNOS expression is significantly correlated with MIB-1 expression.
出处
《第四军医大学学报》
北大核心
2004年第24期2280-2283,共4页
Journal of the Fourth Military Medical University
