摘要
目的 建立稳定表达野生型DNA聚合酶 β的细胞系。方法 将已构建的野生型DNA聚合酶 β的真核表达载体用脂质体法转染中国仓鼠卵巢细胞 ,经G4 18筛选后得到稳定转染的细胞株 ,用免疫印记方法鉴定野生型DNA聚合酶β蛋白水平的表达。 结果 成功转染和筛选出表达外源性DNA聚合酶 β基因的中国仓鼠卵巢细胞株 ,并检测到该基因蛋白水平的高表达。结论 建立稳定表达DNA聚合酶β的细胞株 。
Objective To establish a cell line expressing wild type DNA polymerase β (DNA pol β) stably.Methods The constructed eukaryotic expression vector carring DNApol β gene was transfected into Chinese hamster ovary cell (CHO) with the method of lipofectamine. The stable transfectants were sceened by G418. Expressing wild-type pol β protein was analyzed by Western blot. Results DNA pol β gene was transfected successfully.DNApol β protein was expressed in the transfected CHO cells.Conclusion A cell line stably expressing wild type pol β is established, and it can be used for the further study of gene function and the affection of overexpression of poi β on eukaryotic cell growth.
出处
《河南职工医学院学报》
2004年第4期323-325,共3页
Journal of Henan Medical College For Staff and Workers
