摘要
目的 探讨 SD大鼠重症肌无力 ( myasthenia gravis,MG)中枢神经系统 ( CNS)损害模型大脑皮质基因表达的差异 ,为深入研究 MG的 CNS损害机制提供实验依据。方法 将从 MG患者和非 MG患者血清中提取的免疫球蛋白分别注入实验组和对照组 SD大鼠侧脑室 ,3周后取新鲜脑组织提取 m RNA并分析基因表达的差异 ,将所得的差异表达基因在 Gene Bank中分析。结果 在 2 0 0 0条目的基因中共发现差异表达基因 46条 ,包括全长表达基因 1 7条 ,表达序列标识 2 9条。其中 42条表达减少 ,4条表达增加。差异表达基因包括信号转导相关基因、氨基酸及微量元素代谢相关基因、蛋白转运相关基因、细胞周期相关基因、细胞骨架和运动蛋白基因、免疫相关基因、蛋白质修饰相关基因、DNA结合蛋白及转录起始因子基因、离子通道相关基因等。结论 多种基因与 MG CNS损害相关 ,差异基因表达的检测结果可为深入研究 MG的发病机制提供新思路。
Objective To seek experimental evidence for the pathogenesis of the dysfunction of central nervous system (CNS) in myasthenia gravis (MG) by analyzing the gene expression of the pallium in experimental autoimmune myasthenia gravis models with dysfunction of CNS in SD rats. Methods The immunoglobulin derived from the sera of the MG patients and those of the healthy were injected into the lateral ventricles of the experimental SD rats and the control group respectively. The mRNA from the fresh pallium of all rats was taken for cDNA microarray three weeks later. The genes with obviously variant expression were analysed in GeneBank database. Results Among 2000 genes there were 46 differences including 17 complete gene sequences and 29 ESTs, of which 42 expressed higher and 4 lower in the experimental pallium than the controlled group. The genes with obviously variant expression include signal transduction related genes, metabolism of amino acid or microelement related genes, protein transportation related genes, cell division cycle related genes, cytoskeleton and motor protein genes, immune related genes, modification of protein related genes, DNA binding protein or initiation of transcription related genes and ion channel related genes. Conclusions Many genes are involved in the onset of CNS dysfunction in MG.
出处
《中国神经免疫学和神经病学杂志》
CAS
2004年第4期193-196,共4页
Chinese Journal of Neuroimmunology and Neurology
基金
国家自然科学基金资助项目 ( 3 0 2 70 485 )
关键词
重症肌无力
中枢神经系统
基因芯片
基因表达
myasthenia gravis
central nervous system
cDNA microarray
gene expression
