摘要
目的 :探讨马鞭草及米非司酮抗早孕的细胞学作用机理 ,初步确定马鞭草抗生育的有效部位。方法 :实验选用早孕人工流产蜕膜组织进行体外培养 ,观察马鞭草提取液A、B、C、D及米非司酮对蜕膜细胞形态 ,增殖 ,细胞凋亡及细胞周期动力学的影响。结果 :高于 12 .5mg/ml的A、B、C及 80 μg/ml的米非司同酮均可明显改变蜕膜基质细胞 (DSC)的形态 ,并对其增殖产生显著的抑制作用。 2 5mg/ml的A、B、C及米非司酮(80 μg/ml) 均促进细胞凋亡 ,对细胞周期无明显影响。D均无明显作用。结论 :马鞭草、米非司酮均有抗早孕作用。抑制蜕膜细胞生长 。
To explore machanism of Verbena offcinalis L. and mefepristone with anti-early pregnancy activity on cell level and to investigate the active portion in the Verbena of offcinalis on anti-early pregnancy activity .In this experiment,A,B,C and D were extrated by different ways respectively.By means of cell culture technique,the effect of \%Verbena of offcinalis L\% and mefepristone on the morphology and viability,proliferation,apoptosis and cellcycle proliferation was greatly changed and the viability was significantly inhibited when the decidual cells were preincubated with A,B,C(>12.5mg/ml)and mefepristone(80 μg/ml).The higher the concentration of A,B,C was the more significant inhibition was.A,B,C(25 mg/ml) and Mifeprestone(80 μg/ml) could enhence apotosis in decidual cell,but had no significant effect on cellcycle.D had no obvious effect in above aspects.In conclusion,\% Verbena offcinalis L\% and mefepristone can terminate early pregnancy by inhibiting proliferation and enhancing apoptosis of decidual stromal cells.
出处
《中国天然药物》
SCIE
CAS
CSCD
2004年第4期242-246,共5页
基金
卫生部资助课题 (No .92 2 41)~~
