摘要
Aim To develop a simple and sensitive high-performance liquid chromatographicmethod for determination of plasma concentration of cinnamic acid and pharmacokinetic study in ratsafter a single oral dose of traditional Chinese medicinal preparation Zi-Shen pill. Method Plasmasamples were acidified with hydrochloric acid and extracted with ethyl acetate . Cinnamic acid wasdetermined by HPLC using a G_(18) column. A mobile phase ofmethanbl-acetonitrile-water-triethyl-amine (7:22:73 = 0.2, V/V), with the pH adjusted to 4.0 withphosphoric acid, and with a UV detector set at 340 nm. Results The standard curve was linear overthe range of 1.92- 192.0 μg·mL^(-1). The LLOQ was 1.92 μg·mL^(-1) . The RSDs of within-day andbetween-day precision were < 8%. The mean recovery was 82.0% . Conclusion After validation, themethpd has been used to investigate the pharmacokinetic profiles of the traditional Chinesemedicinal preparation Zi-Shen pill.
目的 建立简便的测定大鼠血浆中肉桂酸血药浓度的高效液相色谱法。方法 样品血浆以 1moL·L- 1 HCl酸化 ,用乙酸乙酯萃取 ,取上清液进行分析。色谱条件为 :流动相 :甲醇 乙腈 水 三乙胺 (7∶2 2∶73∶0 2 ,V V) ,磷酸调pH =4 ,流速 :0 9mL·min- 1 ,检测波长 :340nm。结果 肉桂酸的血药浓度标准曲线的线性范围为 1 92 - 192 0 μg·mL- 1 ,其最低定量限为1 92 μg·mL- 1 ,日内及日间RSD均小于 8%。平均回收率为 82 0 %。大鼠口服滋肾丸后在体内的药动学参数为 :Tmax ≈ 0 5h ,Cmax=4 6 6 5± 4 9μg·mL- 1 ,t1 2 =5 4 4h ,ke=0 12 7handAUC0 -t=2 5 6 91(μg·min·mL- 1 ) ,AUC0 -∞ =2 90 5 2 (μg·min·mL- 1 )。
