摘要
目的:建立聚合酶链反应(PCR)用于快速检测梅毒螺旋体。方法:根据梅毒螺旋体47ku膜蛋白基因序列,自行设计筛选一对特异寡核苷酸引物,建立梅毒螺旋体的PCR。同时对其进行实验室评价和临床标本的检测验证。结果:PCR对梅毒螺旋体能产生特异性扩增,片段大小为252bp,而对其他生殖道常见菌及人基因组DNA不能扩增出任何片段。检测65例临床标本,PCR检查阳性54例(83.1%),梅毒螺旋体明胶颗粒凝集试验(TPPA)检查阳性52例(80%),梅毒螺旋体暗视野显微镜检查阳性40例(61.5%)。PCR检测结果,与暗视野显微镜检查结果比较,差异有显著性(P<0.01);与梅毒血清学检查结果比较,差异无显著性(P>0.05)。结论:PCR可特异、快速地检测梅毒螺旋体,并具有较好的敏感性。
Objective: To establish PCR assay for rapid detection of Treponema pallidum. Methods: According to specific sequence of Treponema pallidum gene (tpp47 gene), a pair of specific primers were designed and PCR assay was developed. Also PCR was evaluated by clinical samples. Results: The target DNA of Treponema pallidum was specifically amplified by its specific primers. The amplified DNA fragment of Treponema pallidum was 252bp. The DNA fragments of other microorganisms in the genital tract and the human genome were not amplified by PCR. Sixty-five clinical samples were detected by PCR, TPPA and dark-field microscopy and the positivity rate was 83.1%, 80% and 61.5%, respectively. The result of PCR was compared with the results of dark-field microscopy and there was a statistically significant difference (P < 0.01). When compared with the result of TPPA, there was no statistically significant difference (P > 0.05). Conclusion: The study indicates that the PCR assay can specifically, rapidly detect Treponema pallidum with high sensitivity.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2004年第7期413-415,共3页
Journal of Clinical Dermatology
关键词
梅毒螺旋体
聚合酶链反应
treponema pallidum
polymerse chain reaction
