摘要
运用RAPD分子标记技术分析了广东省水稻纹枯病菌的群体遗传结构.研究结果表明,水稻纹枯病菌存在遗传分化现象(G=0.165 8).分子方差分析表明群体遗传多样性在广东省地区间、地区内以及县市内3个水平分布,群体遗传分化在地区间的水平非常低(ΦST=0.038),地区内的遗传分化程度较高(ΦST=0.240),遗传分化主要来源县市内菌株的遗传变异(ΦST=0.269),占总群体遗传多样性的73.07％.Mantel分析表明地理距离和遗传距离间缺少相关性,群体分化不符合地理距离隔离模式.
The population genetic structure of rice sheath blight fungi (Rhizoctonia solani Ag-1 IA) was analyzed by using Randomly Amplified Polymorphic DNA (RAPD) in Guangdong Province, China. The results showed that the population genetic differentiation was presented within populations (C=0.165 8). A hier-achical analysis of genetic structure analyzed by molecular variance (AMOVA) revealed that genetic diversity was distributed to populations among regions, within the regions and counties, genetic differentiation among populations in region was in a low level (ΦST= 0.038). A high level of genetic differentiation occurred in populations within regions(ΦST = 0.240) and within counties(ΦST=0.269) , and total genetic diversity was mostly attributable to diversity within populations in counties. The hypothesis of isolation-by-distance in populations of Rhizoctortia solani AG-1 IA in Guangdong was rejected by computing Mantel correlation coefficient between genetic and geographic distance matrices.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2004年第3期43-47,共5页
Journal of South China Agricultural University
基金
国家自然科学基金资助项目(39870438)
广东省自然科学基金资助项目(33985)
关键词
广东
水稻
纹枯病菌
群体遗传结构
分子方差分析
RAPD分子标记
遗传分化
rice sheath blight fungi (Rhizoctonia solani AG-1 LA)
population structure
genetic differentiation
analyses of molecular variance
