摘要
目的：了解亚胺培南对鲍氏不动杆菌,阴沟肠杆菌、铜绿假单胞菌、以及产ESBLs的大肠埃希菌和肺炎克雷伯菌等常见革兰阴性杆菌透导性AmpC酶的发生率。方法：采用底物协同—拮抗法(MSSAT)检测大肠埃希菌,肺炎克雷伯菌,铜绿假单胞菌,鲍氏不动杆菌和阴沟肠杆菌产AmpC酶的情况及构成。结果：产ES- gLs的大肠埃希菌和肺炎克雷伯菌、铜绿假单胞菌、阴沟肠杆菌、鲍氏不动杆菌AmpCs酶的检出率分别为20．7%,23．1%,76%,75%,87．9%；高诱导型AmpC酶的检出率分别为0,15．4%,44%,35%,9．1%；部分去阻遏型AmpC酶的检出率分别为3．5%,7．7%,16%,30%,15．2%；完全去阻遏型AmpC酶的检出率分别为17．2%,0%,16%,10%,63．6%。结论：亚胺培南对铜绿假单胞菌和阴沟肠杆菌产AmpC酶具有高度诱导性,而对产ESBLs大肠埃希菌和肺炎克雷伯菌及鲍氏不动杆菌诱导性低,应引起临床和实验室的重视；AmpC酶的诱导表型与菌株的耐药程度相关。
Objective:To investigate the production of AmpCs induced by imipenem in common gram negative bacilli such as Acinetobacter sp,E.cloacae,P.aeruginosa,and ESBLs produducing strains including E.coli and K.pneumonia.Methods:The production of AmoCs strains of E.coli,K.pneumoniae Acinetobacter,E.cloacae, P.aeruginosa were detected with muti substrates synergy-antagonize test (MSSAT).Results:The positive rates of AmpCs in P.aeruginosa,Acinetobacter,E.cloacae,E.coli and K.pneumonia were 20.7%,23.1%,76%, 75% and 87.9%,respectively.The positive rates of highly inducible AmpCs were 0,15.4%,44%,35% and 9. 1%,respectively.The positive rates of partially derepressed Ampcs were 3.5%,7.7%,16%,30% and 15.2%, respectively.The detecting rates of stably derepressed Ampcs were 17.2%,0,16%,10%,and 63.6%,respec- tively.Conclusion:The results suggested that imipenem showed highly inducible effect for AmpC in E.cloacae and P.aeruginosa,but weak effect in E.coli,K.pneumoniae and Acinetobacter,and the AmpCs inducible phe- nocopy of strains were related to antimimicrobial resistance.This phenomenon should to be appreciated by labora- tory and clinical worker.
出处
《感染．炎症．修复》
2005年第4期216-218,共3页
Infection Inflammation Repair
