摘要
目的研究苦参碱(matrine)对体外培养的人卵巢癌细胞OV2008增殖的影响及相关机制。方法采用四甲基偶氮唑蓝(MTT)比色法检测不同浓度(0、0.125、0.25、0.5、1.0、2.0、4.0 mg/mL)的苦参碱在不同的时间点(24、48、72 h)对OV2008细胞增殖的影响;采用流式细胞术(FCM)检测不同浓度的苦参碱作用于OV2008细胞48 h后细胞周期分布、凋亡率及Fas、Bcl-2蛋白的表达水平。结果苦参碱对OV2008细胞的增殖具有明显的抑制作用,与对照组相比具有显著性差异(P<0.05或P<0.01),并呈现时间和剂量依赖性;细胞周期分析显示,随着苦参碱浓度的增高,G0/G1期细胞比例逐渐增加,S期细胞比例减小,G2/M期细胞比例无明显变化;苦参碱能够诱导细胞凋亡,且凋亡率随着苦参碱浓度的增高而升高(P<0.01);苦参碱能够上调Fas蛋白的表达(P<0.05或P<0.01),下调Bcl-2蛋白的表达(P<0.01)。结论苦参碱可显著抑制OV2008细胞的增殖,且抑制作用呈剂量和时间依赖性。苦参碱对OV2008细胞的增殖抑制作用可能与其诱导细胞周期阻滞及凋亡有关,而苦参碱诱导凋亡可能与其上调Fas蛋白表达、下调Bcl-2蛋白表达有关。
Objective To study the effects of matrine on proliferation of human ovarian cancer OV2008 cells in vitro cell culture and the action mechanism.Methods 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） colorimetric assay was used to examine the effects of matrine at different concentrations（0,0.125,0.25,0.5,1.0,2.0 and 4.0 mg/mL） and different time points（24,48 and 72 h） on proliferation of OV2008 cells.Flow cytometry（FCM） was used to analyze cell cycle,apoptosis rate as well as expression levels of Fas and Bcl-2 proteins of OV2008 cells treated with matrine at different concentrations for 48 h. Results The results showed that compared with the control group,matrine could significantly inhibit the proliferation of OV2008 cells in time-and dose-dependant manners（P〈0.05 or P〈0.01）.Cell cycle analysis indicated that the percentage of OV2008 cells was increased in G0/G1 phase and decreased in S phase with the concentration of matrine increased;there was no obvious change in G2/M phase.The results also indicated that matrine could induce apoptosis of OV2008 cells and the apoptosis rate was increased with the increase of matrine concentration compared with the control group（P〈0.01）.Matrine also up-regulated the expression of Fas protein（P〈0.05 or P〈0.01） and down-regulated the expression of Bcl-2 protein（P〈0.01）.Conclusion Matrine can effectively inhibit the proliferation of OV2008 cells in time-and dose-dependent manners.The inhibition may be related to the arrest of cell cycle and induction of apoptosis.The matrine-induced apoptosis might be related to the up-regulation of Fas protein and down-regulation of Bcl-2 protein.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2010年第5期621-624,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
兰州大学医学科研基金(No.LZUYX200737)~~
关键词
苦参碱
卵巢癌
细胞周期
凋亡
matrine
ovarian carcinoma
cell cycle
apoptosis
