摘要
目的研究大肠埃希菌、肺炎克雷伯菌连续分离株质粒AmpC基因情况和耐药性。方法应用聚合酶链反应(PCR)法对耐药的大肠埃希菌和肺炎克雷伯菌连续分离株进行AmpC酶DHA和ACT-1基因检测和分析。结果68株大肠埃希菌中ACT-1基因阳性4株(5.9%),DHA基因全为阴性;44株肺炎克雷伯菌中DHA基因阳性4株(9.1%),ACT-1基因全为阴性。结论质粒型AmpC酶基因可通过转化、接合等方式转移给其他同种或不同种菌,易于传播,应加强监测。
Objective To study the expression and resistance of plasmid-mediated AmpC gene in continuous isolates from Escherichia coli and Klebsiella pneumoniae. Methods The DHA and ATC-1 types of AmpC enzymes in continuous isolates from Escherichia coli and Klebsiella pneumoniae were detected by polymerase chain reaction(PCR). Results In 68 isolates Escherichia coli, there are 4 isolates, whose ATC-1 types were positive, and the DHA types were all negative. In 44 isolates Klebsiella pneumoniae, there are 4 isolates, whose DHA types were positive, and ATC-1 types were all negative. Conclusions Plasmid-mediated AmpC lactamase gene can be transferred to allogenic and xenogenic bacteria by conversion and conjugation. So they should be under careful surveillance.
出处
《现代实用医学》
2006年第11期777-779,共3页
Modern Practical Medicine
关键词
头孢菌素酶
基因
大肠杆菌
克雷伯菌
肺炎
Cephalosporinase
Gene
Escherichia coli
Klebsiella pneumoniae
