摘要
建立一种抗除草剂转基因稻米制品的四重PCR检测方法.以抗除草剂转基因稻米SPS(sucrose phosphate synthase,蔗糖磷酸合酶基因)内源基因、CAMV35S(Cauliflower mosaic virus 35S,花椰菜花叶病毒35S)启动子、Bar(blalaphos resistance,抗草丁膦除草剂)外源基因、NOS(nopaline synthase,胆脂碱合酶基因)终止子为模板设计四对引物,通过引物浓度和退火温度的优化建立抗除草剂转基因稻米的四重PCR检测方法,以用于转基因甜米酒的检测.结果表明:建立的四重PCR检测方法快速可靠、灵敏准确、特异性好,且操作简便、成本低,可用于市场中抗除草剂转基因稻米及其米制品的快速检测.
s In order to establish a multiplex PCR detection method for herbicide-tolerant transgenic rice and its products,four pairs of primers for the amplification of sucrose phosphate synthase(SPS)gene,Cauliflower mosaic virus 35S(CAMV35S)promoter,blalaphos resistance(Bar)gene and nopaline synthase(NOS)terminator were designed.A multiplex PCR detection method for herbicide-tolerant transgenic sweet rice wine was successfully established through optimizing primer concentration and annealing temperature.The newly developed multiplex PCR method had high efficiency and specificity,and can be used for the fast detection of herbicide-tolerant transgenic rice and its rice products in the market.
作者
宋尚新
邱良焱
高峰
周光宏
肖红梅
SONG Shang-xin;QIU Liang-yan;GAO Feng;ZHOU Guang-hong;XIAO Hong-mei(Key Laboratory of Meat Processing and Quality Control,Ministry of Education,Key Laboratory of Agricultural and Animal Products Processing and Quality Control,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第S02期66-70,共5页
Food Science
基金
农业部转基因生物新品种培育科技重大项(2009ZX08012-014B)
