摘要
本文报告用扫描电镜观察体外培养的人胎儿心肌细胞发育的形态特征。材料来自妊娠10、13、16周人胎儿心脏。首先进行组织块培养,然后观察由培养团块上新生并脱离团块贴璧生长的心肌细胞。培养早期(脱落贴壁生长1周左右),细胞呈球形、短梭形,表面有各种突起。中期(脱落生长约2~4周)细胞呈扁平不规则形。细胞间由突起相连接。晚期(脱落生长超过1个月)部分细胞可发育成带状、柱状,由闰盘样结构连接成分支状。体外培养的胎儿心肌细胞,在发育过程中,可见闰盘样结构形成的过程,通过这种连接,使一群细胞相互连接,平行排列成束,近似于成熟心肌细胞的形态及排列。本文研究提示,体外培养的人胎儿心肌细胞似有分化、发育成熟的趋向。
Morphological features of the developing human fetal cardiac muscle cells in culture was observed with scanning electron microscope.Myocardial tissue masses were isolated from three human fetus of 10,13 and 16 week’s gestation.They were cultured in vitro.New cardiac muscle cells grew and detached from the tissue masses.In the early stage(about one week)the cardiac muscle cells which detached from the tissue masses and grew on the glass slide were spherical or short spindle in shape.There were various processes on the surface of the cells.In the middle stage(about two to four weeks)the cells became irregular in shape.The processes extended from the surface of the cells and formed the intercellular connection.In the later stage(more than one month)part of the cells in culture developed and appeared rod shape.The intercellular connection was composed of the intercalated disc-like structure,the rod-shaped cells joined together and formed branched structures.In the development of human fetus in culture a continuous process of the formation of the intercalated disc-like structure was observed.It was found that the developed cells were gradually arranged in bundles running parallel to their long axis.This kind of structure looked like normal mature morphological feature of cardiac muscle cells.It is therefore considered that the cardiac muscle cells in culture may have possibility to differentiate into mature cardiac muscle.
作者
杨佩荪
高进
Yang Peisun;Gao Jin(Department of Pathology,Capital Institute of Medicine,Beijing;Basic Medical Science Institute,Chinese Academy of Medicine,Beijing)
出处
《解剖学报》
CAS
1987年第1期87-90,129,共5页
Acta Anatomica Sinica
关键词
人胎儿心脏
组织培养
心肌细胞发育
闰盘形成
扫描电镜
Human fetal heart
Tissue culture
Myocardial development
Intercalated disc
Scanning electron microscopy
