摘要
目的研究大蒜与含氯消毒剂联用对多重耐药鲍曼不动杆菌体外抑菌实验。方法选取由医院检验科2017年3月—2019年2月从临床标本中获得的80株多重耐药鲍曼不动杆菌,采用随机法将其分为大蒜素、含氯消毒剂、大蒜素与含氯消毒剂联合用药组(以下简称联合用药组)各26例,将其试用于单一用药组和联合用药组对比研究分析结果,比较分析用药后单一用药组和联合用药组MIC值及协同、累加、拮抗作用等指标分析。结果最高菌株检出率为呼吸道菌株35例(43.75%),伤口分泌物菌株临床收集18例(22.5%);消化道菌株17例(21.25%),其他检出率为10例(12.5%)。联合用药产生协同作用(42例,占53%),累加作用(25例,占31%),无关作用(13例,占16%),拮抗作用(0例)。联合用药组抑制细菌生长所需药物的最低浓度MIC[32.0~512.0μg/mL]明显低于单一用药组大蒜素[64.0~1024.0μg/mL]、含氯消毒剂[512.0~2048.0μg/mL],比较差异有统计学意义(P<0.05);联合用药组MIC50(256.0μg/mL)明显低于单一用药组大蒜素MIC50(512.0μg/mL)、含氯消毒剂MIC50(1024μg/mL)比较差异有统计学意义(P<0.05);联合用药组MIC90(512.0μg/mL)明显低于单一用药组大蒜素MIC90(1024.0μg/mL)、含氯消毒剂MIC90(2048.0μg/mL),比较差异有统计学意义(P<0.05)。结论大蒜和含氯消毒剂联合用药对多重耐药鲍曼不动杆菌的抑菌作用比单一药物作用时抑菌效果更好,大蒜作为广谱的绿色抗生素与常用化学消毒剂(含氯消毒剂)在体外联用,对抑制多重耐药鲍曼不动杆菌具有协同和累加作用,可以改善多重耐药鲍曼不动杆菌的感染,减少其他抗生素使用,具有良好的开发价值。
Objective To study the in vitro bacteriostatic experiment of garlic and chlorine-containing disinfectant against multidrug-resistant Acinetobacter baumannii.Methods Eighty strains of multi-drug-resistant Acinetobacter baumannii obtained from clinical specimens in our laboratory from March 2017 to February 2019 were selected and randomly divided into allicin,chlorine-containing disinfectant,and garlic combined with chlorine-containing disinfectant group(hereinafter referred to as the combined drug group),26 cases in each group.The MIC values,synergy,accumulation,antagonism and other indicators of the single drug group and the combined drug group after drug administration were compared and analyzed.Results The highest detection rate of strains was 35 cases of respiratory strains(43.75%),18 cases of wound secretion strains were clinically collected(22.5%),17 cases of digestive tract strains(21.25%),and the other detection rates were 10 cases(12.5%).The combined medication produced synergistic effects(42 cases,53%),cumulative effects(25 cases,31%),unrelated effects(13 cases,16%)and antagonistic effects(0 case).The minimum concentration MIC(32.0~512.0μg/mL)of the drug required for the combined drug group to inhibit bacterial growth was significantly lower than that of the allicin group(64.0~1024.0μg/mL)and the chlorine-containing disinfectant(512.0~2048.0 mg/mL).The difference was statistically significant(P<0.05).The MIC50(256.0μg/mL)of the combined drug group was significantly lower than that of the allicin MIC50(512.0μg/mL)and the chlorine-containing disinfectant MIC50 of the single drug group(1024μg/mL).There was a statistically significant difference(P<0.05).MIC90(512.0μg/mL)in the combination group was significantly lower than that of the allicin MIC90(1024.0μg/mL)and chlorine-containing disinfectant MIC90(2048.0μg/mL)in the single-agent group.The difference was statistically significant(P<0.05).Conclusion The combined effect of garlic and chlorine-containing disinfectant on multidrug-resistant Acinetobacter baumannii is better than that of single drug.Garlic is a broad-spectrum green antibiotic and commonly-used chemical disinfectant(chlorine-containing disinfection).Agent in combination in vitro,has a synergistic and cumulative effect on the inhibition of multidrug-resistant Acinetobacter baumannii,can improve the infection of multidrug-resistant Acinetobacter baumannii,reduce the use of other antibiotics and has good development value.
作者
谭晓宇
凌寿坚
钟一梅
TAN Xiaoyu;LING Shoujian;ZHONG Yimei(Wuyi Hospital of Traditional Chinese Medicine of Jiangmen,Jiangmen 529000,Guangdong,China)
出处
《辽宁中医杂志》
CAS
2021年第7期179-182,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
江门市医疗卫生领域科技计划项目(2018020400720004074)
关键词
多重耐药鲍曼不动杆菌
大蒜素
含氯消毒剂
体外抑菌实验
multidrug-resistant Acinetobacter baumannii
allicin
chlorine-containing disinfectant
in vitro bacteriostatic experiment
