摘要
目的探讨移植脂肪间充质干细胞(adipose-derived stem cells,ADSC)转化神经元移植对颞叶癫疒间大鼠学习记忆的影响。方法SD大鼠按实验要求,随机分成4组:移植组(n=15,颞叶癫疒间大鼠造模后在海马植入ADSC转化神经元),假移植组(n=15,颞叶癫疒间大鼠造模后在海马注射生理盐水),NS组(n=10,实验大鼠不造模,仅在海马注射生理盐水)和ADSC对照组(n=10,实验大鼠不造模,仅在海马植入ADSC转化神经元)。移植前、移植后2周、移植后2个月,分别进行Morris水迷宫实验,比较大鼠学习记忆能力的变化。结果移植后2周及2个月,与假移植组相比,移植组大鼠寻找平台平均潜伏期均缩短(P<0.05、P<0.01);而与NS组及ADSC对照组相比,无显著性差异(均P>0.05)。移植后2周及2个月,与NS组比较,移植组和ADSC对照组大鼠穿越Ⅱ象限时间,均无显著性差异(均P>0.05);而与移植组比较,假移植组大鼠穿越Ⅱ象限时间明显缩短(P<0.01)。结论ADSC转化神经元移植能改善颞叶癫疒间大鼠的学习记忆能力,且无明显副作用,具体机制尚待进一步研究。
Objective To investigate the effects oftransplantation ofadipose-derived stem cells(ADSCs)transformed neurons on the learning and memory of temporal lobe epilepsy rats.Methods The SD rats were randomly divided into 4 groups according to the experimental requirements:transplantation group(n=15,ADSC-transformed neurons were implanted into the hippocampus after modeling of temporal lobe epilepsy rats),sham-transplantation group(n=15,temporal lobe epilepsy rats after modeling,saline was injected into the hippocampus),NS group(n=10,experimental rats were not modeled,saline was injected only in the hippocampus)and ADSC control group(n=10,experimental rats were not modeled,only ADSC-transformed neurons were implanted into the hippocampus).Before transplantation,2 weeks and 2 months after transplantation,Morris water maze tests were performed to compare the changes in learning and memory abilities of rats.Results After 2 weeks and 2 months of transplantation,compared with the sham-transplantation group,the average latency of finding platform in the transplantation group was shorter(P<0.05,P<0.01);compared with the NS group and the ADSC control group,there was no significant difference(both P>0.05).After 2 weeks and 2 months of transplantation,compared with the NS group,there was no significant difference in the time across quadrantⅡbetween the transplantation group and the ADSC control group(both P>0.05);compared with the transplantation group,the time across quadrantⅡof the rats in the sham-transplantation group was significantly shorter(P<0.01).Conclusions The ADSC-transformed neuron transplantation can improve the learning and memory ability of temporal lobe epilepsy rats without significant side effects.The specific mechanism needs further study.
作者
王丽丽
梅广海
张宁
王桂松
赵迎春
潘晓春
陈红专
朱亮
Wang Lili;Mei Guanghai;Zhang Ning;Wang Guisong;Zhao Yingchun;Pan Xiaochun;Chen Hongzhuan;Zhu Liang(Department of Neurology,Songjiang Branch of the First People's Hospital Affiliated to Shanghai Jiaotong University,Shanghai 201609,China;Department of Neurosurgery,Huashan Hospital Affiliated to Shanghai Hehai University,Shanghai 200040,China;Department of Neurosurgery,South Hospital of the Sixth People's Hospital Affiliated to Shanghai Jiaotong University,Shanghai 201499,China;Department of Neurosurgery,Renji Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200127,China;Laboratory of Pharmacology,Basic Medical College,Shanghai Jiaotong University,Shanghai 200025,China)
出处
《中国微侵袭神经外科杂志》
CAS
2020年第2期80-84,共5页
Chinese Journal of Minimally Invasive Neurosurgery
