目的:外周免疫景观可能在肝细胞癌(HCC)的识别和控制中起关键作用。本研究旨在探讨外周血CD8+T细胞数量在预测肝细胞癌根治性肝切除术后预后中的价值。方法:选取2018年8月至2023年7月79例于我院行根治性肝切除术的初诊HCC患者作为研究对...目的:外周免疫景观可能在肝细胞癌(HCC)的识别和控制中起关键作用。本研究旨在探讨外周血CD8+T细胞数量在预测肝细胞癌根治性肝切除术后预后中的价值。方法:选取2018年8月至2023年7月79例于我院行根治性肝切除术的初诊HCC患者作为研究对象,分析外周血CD8+T细胞数量对患者预后的预测价值。流式细胞术检测CD8+T细胞数量。结果:CD8+T细胞数量在早期复发和死亡患者中明显减少(p Cox回归分析显示CD8+T细胞数量是术后预后的独立影响因素(p Objective: The peripheral immune landscape may play a key role in determining hepatocellular carcinoma (HCC) recognition and control. This study aimed to examine the utility of peripheral blood CD8+T cells in predicting prognosis in HCC after curative hepatectomy. Methods: 79 newly diagnosed HCC patients from August 2018 to July 2023 were selected as research objects, we analyzed the prognostic value of peripheral blood CD8+T cells of HCC patients who underwent curative hepatectomy. The number of CD8+T cells was detected by flow cytometry. Results: The number of CD8+T cells was significantly decreased in patients who developed recurrence and death (all p Cox regression demonstrated that the number of CD8+T cells was an independent indicator for poor prognosis after hepatectomy (both p < 0.050). The recurrence-free survival (RFS) and overall survival (OS) in decrease group were significantly shorter than non-decrease group. The results were confirmed by the subgroup analysis. Conclusion: The number of CD8+T cells in peripheral blood is associated with dismal outcomes in HCC patients and can serve as a novel prognostic indicator for HCC patients after curative hepatectomy.展开更多
文摘目的:外周免疫景观可能在肝细胞癌(HCC)的识别和控制中起关键作用。本研究旨在探讨外周血CD8+T细胞数量在预测肝细胞癌根治性肝切除术后预后中的价值。方法:选取2018年8月至2023年7月79例于我院行根治性肝切除术的初诊HCC患者作为研究对象,分析外周血CD8+T细胞数量对患者预后的预测价值。流式细胞术检测CD8+T细胞数量。结果:CD8+T细胞数量在早期复发和死亡患者中明显减少(p Cox回归分析显示CD8+T细胞数量是术后预后的独立影响因素(p Objective: The peripheral immune landscape may play a key role in determining hepatocellular carcinoma (HCC) recognition and control. This study aimed to examine the utility of peripheral blood CD8+T cells in predicting prognosis in HCC after curative hepatectomy. Methods: 79 newly diagnosed HCC patients from August 2018 to July 2023 were selected as research objects, we analyzed the prognostic value of peripheral blood CD8+T cells of HCC patients who underwent curative hepatectomy. The number of CD8+T cells was detected by flow cytometry. Results: The number of CD8+T cells was significantly decreased in patients who developed recurrence and death (all p Cox regression demonstrated that the number of CD8+T cells was an independent indicator for poor prognosis after hepatectomy (both p < 0.050). The recurrence-free survival (RFS) and overall survival (OS) in decrease group were significantly shorter than non-decrease group. The results were confirmed by the subgroup analysis. Conclusion: The number of CD8+T cells in peripheral blood is associated with dismal outcomes in HCC patients and can serve as a novel prognostic indicator for HCC patients after curative hepatectomy.
文摘目的研究长链非编码RNA(long non-coding RNA,LncRNA)LINC01137在非小细胞肺癌(nonsmall cell lung cancer,NSCLC)免疫逃逸中的生物学功能及其潜在的调节机制。方法采集24例健康志愿者和24例NSCLC患者血液样本,并收集NSCLC肿瘤组织和癌旁组织检测LINC01137水平。利用Starbase数据库预测LINC01137与miR-22-3p的结合位点,荧光素酶报告基因分析进行验证。采用A549细胞来源的外泌体和/或sh-LINC01137干扰序列转染A549细胞,检测细胞增殖和侵袭能力;收集转染后的细胞上清液培养CD8^(+)T细胞,检测CD8^(+)T细胞耗竭标志物干扰素-γ(interfereron-γ,IFN-γ)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、颗粒霉素B(granzyme B)和白细胞介素-2(interleukin-2,IL-2)水平,以及PD-1+Tim3^(+)CD8^(+)T细胞百分比。采用外泌体和/或miR-22-3p模拟物(miR-22-3p mimic)转染CD8^(+)T细胞,检测PD-1蛋白水平。结果与癌旁组织相比,NSCLC肿瘤组织中LINC01137表达(3.357±0.548 vs 1.011±0.371)明显升高;与健康志愿者相比,NSCLC患者外周血LINC01137表达(3.216±0.342 vs 1.007±0.313)亦明显升高,差异具有统计学意义(t=-17.367,-17.147,均P<0.001)。肿瘤组织LINC01137表达与外周血中LINC01137表达呈正相关(r=0.755,P<0.05)。在A549细胞来源的外泌体中LINC01137显著富集。与Exo+sh-NC组相比,Exo+sh-LINC01137组细胞活力(65.852%±4.715%vs 100.153%±11.934%)及细胞侵袭(21.464%±3.481%vs 43.126%±1.447%)能力显著降低,差异具有统计学意义(t=4.630,9.953,均P<0.01)。NSCLC患者外周血中LINC01137表达和CD8^(+)T细胞百分比呈负相关(r=-0.520,P<0.05)。与Exo+sh-NC组相比,Exo+sh-LINC01137组IFN-γ(3865.314±543.852 pg/ml vs 1786.971±105.982 pg/ml),TNF-α(4631.930±510.715pg/ml vs 1973.242±379.623pg/ml),Granzyme B(3876.496±312.438pg/ml vs 1879.439±287.584pg/ml)和IL-2 mRNA水平(3.286±0.437 vs 1.015±0.314)升高,PD-1+Tim3^(+)CD8^(+)T细胞百分比(7.680%±2.185%vs 18.952%±3.216%)降低,差异具有统计学意义(t=-6.497,-7.237,-8.146,-7.310,5.021,均P<0.01)。miR-22-3p是LINC01137的靶基因。与Exo+NC mimic组相比,Exo+miR-22-3p组PD-1蛋白水平(0.384±0.087 vs 1.003±0.147)显著降低,差异有统计学意义(t=6.277,P<0.01)。结论NSCLC患者肿瘤组织及外周血中LINC01137表达显著上调;NSCLC细胞来源的外泌体中LINC01137通过靶向CD8^(+)T细胞中miR-22-3p并抑制其表达,诱导CD8^(+)T细胞耗竭,促进NSCLC细胞免疫逃逸。
