摘要
目的研究在肝细胞癌(hepatocellutar carcinoma,HCC)中唾液酸免疫球蛋白型凝集素-15(Sialic acid immunoglobulin type lectin 15,Siglec-15)与CD4^(+)、CD8^(+)的表达及相关性,并分析其与临床病理特征及预后的影响。方法通过TCGA数据库分析HCC与正常组织中Siglec-15、CD4^(+)T、CD8^(+)T mRNA的表达水平。收集我院2019年1月至2022年1月行肝癌根治切除术的27例新鲜HCC组织及正常组织标本和56例HCC组织及正常组织石蜡切片标本,qRT-PCR检测Siglec-15和CD4^(+)T、CD8^(+)T mRNA表达水平,IHC检测Siglec-15和CD4^(+)、CD8^(+)蛋白表达水平,并对Siglec-15与CD4^(+)、CD8^(+)蛋白之间的关系及临床病理因素和预后进行分析。结果HCC患者中Siglec-15在HCC组织表达高于正常组织(P<0.05),CD4^(+)、CD8^(+)在HCC组织表达低于正常组织(P<0.05);在HCC组织中,Siglec-15表达与CD4^(+)、CD8^(+)蛋白表达呈负相关(P<0.05)。在HCC组织中,Siglec-15蛋白的表达与患者的TNM分期、肿瘤大小相关(P<0.05),CD4^(+)蛋白的表达与患者的肿瘤大小相关(P<0.05),CD8^(+)蛋白的表达与患者的TNM分期、远处转移相关(P<0.05)。Siglec-15及CD4^(+)的表达与患者的无进展生存期具有相关性(P<0.05)。结论HCC组织中Siglec-15高表达、CD4^(+)、CD8^(+)低表达,且Siglec-15与CD4^(+)、CD8^(+)表达呈负相关。Siglec-15及CD4^(+)的表达高低与HCC患者进展及不良预后相关,可作为预测HCC患者的独立预后因素。
Objective To investigate the expression and correlation between Siglec-15 and CD4^(+),CD8^(+)in HCC,and to analyze its influence on clinicopathological features and prognosis.Methods The mRNA expression levels of Siglec-15,CD4^(+)T and CD8^(+)T in HCC tissue and normal tissues were analyzed by TCGA database.Twenty-seven fresh HCC tissue and normal tissue specimens and 56 paraffin sections of HCC tissue and normal tissue were collected from our hospital from Jan.2019 to Jan.2022.qRT-PCR was used to detect the expression of Siglec-15,CD4^(+)T and CD8^(+)T mRNA.IHC was used to detect the expression of Siglec-15,CD4^(+)and CD8^(+).The relationship between Siglec-15 and CD4^(+),CD8^(+)protein level,clinicopathological factors and prognosis were analyzed.Results The expression of Siglec-15 in HCC tissues was higher than that in normal tissues(P<0.05),and the expression of CD4^(+)and CD8^(+)in HCC tissues was lower than that in normal tissues(P<0.05).In HCC,the expression of Siglec-15 was negatively correlated with the expression of CD4^(+)and CD8^(+)protein(P<0.05).In HCC tissues,the expression of Siglec-15 protein was correlated with the TNM stage and tumor size of patients(P<0.05),the expression of CD4^(+)protein was correlated with the tumor size of patients(P<0.05),and the expression of CD8^(+)protein was correlated with the TNM stage and distant metastasis of patients(P<0.05).Siglec-15 and CD4^(+)expression were correlated with progression-free survival(P<0.05).Conclusion Siglec-15 is highly expressed and CD4^(+)and CD8^(+)are low in HCC tissues,and Siglec-15 is negatively correlated with the expressions of CD4^(+)and CD8^(+).The expression levels of Siglec-15 and CD4^(+)are correlated with the progression and poor prognosis of HCC patients,and can be used as independent prognostic factors for HCC patients.
作者
张亚丽
郭彩茹
关璐璐
王金艳
闫家伟
亓民
文学军
ZHANG Yali;GUO Cairu;GUAN Lulu;WANG Jinyan;YAN Jiawei;QI Min;WEN Xuejun(Xinxiang Medical College,Xinxiang 453003;Department of Geriatric Medicine,Luoyang CentralHospital Affiliated to Zhengzhou University;Luoyang Key Laboratory of Individualized Treatment ofLiver Cancer,China;Key Laboratory of Regenerative Medicine,University of Virginia,USA)
出处
《胃肠病学和肝病学杂志》
2025年第1期95-101,共7页
Chinese Journal of Gastroenterology and Hepatology
基金
省卫健委(联合共建)项目(LHGJ20220955)。
