目的:探讨藿香正气液(HXZQ)对脂多糖(LPS)引起的急性肺损伤(ALI)的保护作用及其分子机制。方法:HXZQ灌胃7 d后,采用腹腔注射LPS24 h建立ALI动物模型,观察HXZQ对LPS诱导的ALI的保护作用。结果:HXZQ显著降低中性粒细胞浸润,表现为中性粒...目的:探讨藿香正气液(HXZQ)对脂多糖(LPS)引起的急性肺损伤(ALI)的保护作用及其分子机制。方法:HXZQ灌胃7 d后,采用腹腔注射LPS24 h建立ALI动物模型,观察HXZQ对LPS诱导的ALI的保护作用。结果:HXZQ显著降低中性粒细胞浸润,表现为中性粒细胞活性标志物髓过氧化物酶(MPO)的降低;HXZQ还能抑制巨噬细胞介导的炎症,具体表现为巨噬细胞标志物CD68和促炎因子Il-6、Il-1β的降低;同时,HXZQ通过降低淋巴细胞介导的炎症发挥免疫调节作用,具体表现为细胞因子Il-17a和Gm-csf的降低。结论:HXZQ可以通过抑制免疫细胞的浸润及其分泌的炎症细胞因子来拮抗LPS诱导的ALI。我们的研究强调了HXZQ的免疫调节作用,并提示HXZQ可能是治疗ALI的新药物。Objective: To investigate the protective effect of Huoxiangzhengqi Liquid (HXZQ) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and its molecular mechanism. Methods: After 7d of HXZQ gavage, an animal model of ALI was established by intraperitoneal injection of LPS for 24 h to observe the protective effect of HXZQ on LPS-induced ALI. Results: HXZQ significantly reduced neutrophil infiltration, as evidenced by the reduction of myeloperoxidase (MPO), a marker of neutrophil activity;HXZQ also inhibited macrophage-mediated inflammation, as evidenced by the reduction of the macrophage marker CD68 and the pro-inflammatory factors Il-6 and Il-1β;at the same time, HXZQ played an immunomodulatory role by decreasing lymphocyte-mediated inflammation. This was specifically manifested by the reduction of cytokines Il-17a and Gm-csf. Conclusion: HXZQ can antagonize LPS-induced ALI by inhibiting the infiltration of immune cells and their secretion of inflammatory cytokines. Our study highlights the immunomodulatory effects of HXZQ and suggests that HXZQ may be a new drug for the treatment of ALI.展开更多
随着社会发展,人们精神压力随之增加,失眠、抑郁发病率逐年上升,二者互为因果,失眠抑郁共病的治疗是目前临床需要关注的问题之一。现代医家从安神通阳、疏肝解郁等理论辨治失眠抑郁共病。吴节教授认为失眠抑郁共病之本在于中焦阻滞,肝...随着社会发展,人们精神压力随之增加,失眠、抑郁发病率逐年上升,二者互为因果,失眠抑郁共病的治疗是目前临床需要关注的问题之一。现代医家从安神通阳、疏肝解郁等理论辨治失眠抑郁共病。吴节教授认为失眠抑郁共病之本在于中焦阻滞,肝脾不调,总结出一套从中焦辨治失眠抑郁共病的方法,提出“祛湿邪–调中焦–畅气机–和肝脾”的治则,运用调中理气“脾十针”与藿香正气散加减治疗失眠抑郁共病,取得良好疗效。文章分析总结吴节教授从中焦论治失眠抑郁共病的诊疗思路,以期为临床治疗提供参考。With the advancement of society, people’s mental stress escalates, and the incidence of insomnia with depression rises year by year, mutually causing and influencing each other. The treatment of the comorbidity of insomnia and depression constitutes one of the issues that require attention in the clinical setting. Contemporary physicians differentiate and treat the insomnia with depression and depression based on the theories of calming the vitality and promoting yang, soothing the liver and resolving depression. Dr. Wu contends that the root cause of the comorbidity of insomnia and depression lies in the obstruction of the middle energizer and the abnormalities of the liver and spleen. She formulates a set of approaches to distinguish and treat the comorbidity of insomnia and depression starting from the middle energizer and proposes the treatment principles of “dispelling dampness, regulating the middle energizer, regulating the qi movement, harmonizing the liver and spleen”. She employs the “Spleen Ten Needles” and Huoxiang Zhengqi Powder for treating the comorbidity of insomnia and depression, achieving favorable therapeutic effects. This paper conducts an analysis and summary of Dr. Wu’s diagnosis and treatment of the comorbid diseases of insomnia and depression with a central focus, aiming to offer references for clinical treatment.展开更多
文摘目的:探讨藿香正气液(HXZQ)对脂多糖(LPS)引起的急性肺损伤(ALI)的保护作用及其分子机制。方法:HXZQ灌胃7 d后,采用腹腔注射LPS24 h建立ALI动物模型,观察HXZQ对LPS诱导的ALI的保护作用。结果:HXZQ显著降低中性粒细胞浸润,表现为中性粒细胞活性标志物髓过氧化物酶(MPO)的降低;HXZQ还能抑制巨噬细胞介导的炎症,具体表现为巨噬细胞标志物CD68和促炎因子Il-6、Il-1β的降低;同时,HXZQ通过降低淋巴细胞介导的炎症发挥免疫调节作用,具体表现为细胞因子Il-17a和Gm-csf的降低。结论:HXZQ可以通过抑制免疫细胞的浸润及其分泌的炎症细胞因子来拮抗LPS诱导的ALI。我们的研究强调了HXZQ的免疫调节作用,并提示HXZQ可能是治疗ALI的新药物。Objective: To investigate the protective effect of Huoxiangzhengqi Liquid (HXZQ) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and its molecular mechanism. Methods: After 7d of HXZQ gavage, an animal model of ALI was established by intraperitoneal injection of LPS for 24 h to observe the protective effect of HXZQ on LPS-induced ALI. Results: HXZQ significantly reduced neutrophil infiltration, as evidenced by the reduction of myeloperoxidase (MPO), a marker of neutrophil activity;HXZQ also inhibited macrophage-mediated inflammation, as evidenced by the reduction of the macrophage marker CD68 and the pro-inflammatory factors Il-6 and Il-1β;at the same time, HXZQ played an immunomodulatory role by decreasing lymphocyte-mediated inflammation. This was specifically manifested by the reduction of cytokines Il-17a and Gm-csf. Conclusion: HXZQ can antagonize LPS-induced ALI by inhibiting the infiltration of immune cells and their secretion of inflammatory cytokines. Our study highlights the immunomodulatory effects of HXZQ and suggests that HXZQ may be a new drug for the treatment of ALI.
文摘随着社会发展,人们精神压力随之增加,失眠、抑郁发病率逐年上升,二者互为因果,失眠抑郁共病的治疗是目前临床需要关注的问题之一。现代医家从安神通阳、疏肝解郁等理论辨治失眠抑郁共病。吴节教授认为失眠抑郁共病之本在于中焦阻滞,肝脾不调,总结出一套从中焦辨治失眠抑郁共病的方法,提出“祛湿邪–调中焦–畅气机–和肝脾”的治则,运用调中理气“脾十针”与藿香正气散加减治疗失眠抑郁共病,取得良好疗效。文章分析总结吴节教授从中焦论治失眠抑郁共病的诊疗思路,以期为临床治疗提供参考。With the advancement of society, people’s mental stress escalates, and the incidence of insomnia with depression rises year by year, mutually causing and influencing each other. The treatment of the comorbidity of insomnia and depression constitutes one of the issues that require attention in the clinical setting. Contemporary physicians differentiate and treat the insomnia with depression and depression based on the theories of calming the vitality and promoting yang, soothing the liver and resolving depression. Dr. Wu contends that the root cause of the comorbidity of insomnia and depression lies in the obstruction of the middle energizer and the abnormalities of the liver and spleen. She formulates a set of approaches to distinguish and treat the comorbidity of insomnia and depression starting from the middle energizer and proposes the treatment principles of “dispelling dampness, regulating the middle energizer, regulating the qi movement, harmonizing the liver and spleen”. She employs the “Spleen Ten Needles” and Huoxiang Zhengqi Powder for treating the comorbidity of insomnia and depression, achieving favorable therapeutic effects. This paper conducts an analysis and summary of Dr. Wu’s diagnosis and treatment of the comorbid diseases of insomnia and depression with a central focus, aiming to offer references for clinical treatment.
