Fluorescent nanoparticles have good chemical stability and photostability,controllable optical properties and larger stokes shift.In light of their designability and functionability,the fluorescent nanoparticles are w...Fluorescent nanoparticles have good chemical stability and photostability,controllable optical properties and larger stokes shift.In light of their designability and functionability,the fluorescent nanoparticles are widely used as the fluorescent probes for diverse applications.To enhance the sensitivity and selectivity,the combination of the fluorescent nanoparticles with the molecularly imprinted polymer,i.e.molecularly imprinted fluorescent nanoparticles(MIFN),was an effective way.The sensor based on MIFN(the MIFN sensor)could be more compatible with the complex sample matrix,which was especially widely adopted in medical and biological analysis.In this mini-review,the construction method,detective mechanism and types of MIFN sensors are elaborated.The current applications of MIFN sensors in pharmaceutical analysis,including pesticides/herbicide,veterinary drugs/drugs residues and human related proteins,are highlighted based on the literature in the recent three years.Finally,the research prospect and development trend of the MIFN sensor are forecasted.展开更多
A novel fluorescent probe,6-（N,N-dimethylamino）-2-naphthoylacryl acid（ACADAN） was designed and synthesized as a fluorescent sensor for Cu^2＋ in aqueous media.Significant amplification of fluorescence signals with...A novel fluorescent probe,6-（N,N-dimethylamino）-2-naphthoylacryl acid（ACADAN） was designed and synthesized as a fluorescent sensor for Cu^2＋ in aqueous media.Significant amplification of fluorescence signals without causing any discernible change of maximum fluorescence emission wavelength（λ max） was observed upon the addition of Cu^2＋.Importantly,ACADAN is capable of recognizing Cu^2＋ selectively in aqueous media in the presence of various biologically relevant metal ions and the prevalent toxic metal ions in the environment with high sensitivity（detection limit was 0.1 μmol/L）.展开更多
The survival of human beings is inseparable from the development of agriculture.The use of pesticides is still an important means to ensure agricultural production,economic benefits and the national economy.With the a...The survival of human beings is inseparable from the development of agriculture.The use of pesticides is still an important means to ensure agricultural production,economic benefits and the national economy.With the abuse of pesticides,some pesticides are difficult to degrade after entering the natural world,which leads to many serious problems through the transmission and enrichment of food chains.Pesticide residue detection is a long-term challenge in the field of environment and food safety detection.In recent years,a large number of studies have been carried out on the detection of pesticide residues based on fluorescence sensing methods.This paper focuses on the application of fluorescence sensors in environmental samples or food,and analyzes the advantages and problems of existing fluorescence sensors in design,construction and data processing.Finally,the possible improvement directions in the future are discussed to promote its potential commercial value application research.展开更多
Increasing use of silver in various fields has caused Ag^(+)pollution in water environment,taking great threats to people’s health.As a consequence,establishing rapid and reliable methods for sensitive determination ...Increasing use of silver in various fields has caused Ag^(+)pollution in water environment,taking great threats to people’s health.As a consequence,establishing rapid and reliable methods for sensitive determination of Ag^(+)is of great significance.Fluorescent(FL)sensors based on carbon dots(CDs),an excellent carbonaceous nanomaterial with strong and stable fluorescence,have absorbed extensive attentions in analysis of pollutants due to its advantages of carbon sources being readily available,low cost,easy operation and fast response.Moreover,ion-imprinting is a better way to increase the selectivity of the proposed method.Present work described an effective method for the sensitive measurement of silver ion in water samples in combination with magnetic ion-imprinted solid phase extraction and CDs based fluorescent sensor,which took full advantages of easy separation and high enrichment of magnetic solid phase extraction,high selectivity of ion-imprinting technology,and sensitivity and rapid response of fluorescent sensor from CDs.Sulfur-doped CDs derived from dithizone and magnetic ion-imprinted nanomaterial were prepared,and characterized with Fourier transform infrared spectroscopy and transmission electron microscope,etc.Magnetic Ag^(+)imprinted nanomaterial based solid phase extraction was employed for separating and enriching Ag^(+)from water samples.The significant parameters were optimized in detail.Under the optimal conditions,the proposed method provided good linearity in the range of 0.01-0.4μmol/L and low detection limit of 3 nmol/L.The reliability of the proposed method was validated with real water samples,and the results demonstrated that the proposed method was simple,robust,selective and sensitive detection tool for Ag^(+)in real water samples.展开更多
Benzo[4,5]imidazo[1,2-a]pyrimidine-based derivatives play crucial roles in medicines,pesticides,tracers and photoelectric materials.However,their synthesis approach still needs to be optimized,and their fluorescent pr...Benzo[4,5]imidazo[1,2-a]pyrimidine-based derivatives play crucial roles in medicines,pesticides,tracers and photoelectric materials.However,their synthesis approach still needs to be optimized,and their fluorescent properties in intracellular microenvironment are unclear.Here,a Cu(II)-catalyzed cascade coupling cyclization reaction was successfully developed to synthesize benzo[4,5]imidazo[1,2-a]pyrimidine scaffold with mild reaction conditions,broad substrate scopes and high yields.After a system study,we found that compound 4aa displayed an optimal viscosity-specific response with remarkable fluorescence enhancement(102-fold)for glycerol at 490 nm.Particularly,4aa possessed excellent structure-inherent targeting(SIT)capability for lysosome(P=0.95)with high p H stability and large Stokes shift.Importantly,4aa was validated for its effectiveness in diagnosing lysosomal storage disorders(LSD)in living cells.The 4aa also showed its potential to map the micro-viscosity and its metabolism process in zebrafish.This work not only affords an efficient protocol to fabricate benzo[4,5]imidazo[1,2-a]pyrimidine derivatives,reveals this skeleton has excellent SIT features for lysosome,but also manifests that 4aa can serve as a practical tool to monitor lysosomal viscosity and diagnose LSD.展开更多
The brain has very high energy requirements and consumes 20% of the oxygen and 25% of the glucose in the human body. Therefore, the molecular mechanism under- lying how the brain metabolizes substances to support neur...The brain has very high energy requirements and consumes 20% of the oxygen and 25% of the glucose in the human body. Therefore, the molecular mechanism under- lying how the brain metabolizes substances to support neural activity is a fundamental issue for neuroscience studies. A well-known model in the brain, the astrocyte- neuron lactate shuttle, postulates that glucose uptake and glycolytic activity are enhanced in astrocytes upon neu- ronal activation and that astrocytes transport lactate into neurons to fulfill their energy requirements. Current evidence for this hypothesis has yet to reach a clear consensus, and new concepts beyond the shuttle hypothesis are emerging. The discrepancy is largely attributed to the lack of a critical method for real-time monitoring of metabolic dynamics at cellular resolution. Recent advances in fluorescent protein-based sensors allow the generation of a sensitive, specific, real-time readout of subcellular metabolites and fill the current technological gap. Here,we summarize the development of genetically encoded metabolite sensors and their applications in assessing cell metabolism in living cells and in vivo, and we believe that these tools will help to address the issue of elucidating neural energy metabolism.展开更多
Three novel rhodamine-based Hg^2＋ fluorescent sensors were designed and synthesized. The sensors could work in semi-aqueous solutions with nearly neutral p H and showed high selectivity and sensitivity to Hg^2＋ with...Three novel rhodamine-based Hg^2＋ fluorescent sensors were designed and synthesized. The sensors could work in semi-aqueous solutions with nearly neutral p H and showed high selectivity and sensitivity to Hg^2＋ with remarkable fluorescence enhancement. For these three sensors, the linear working range broadened（0–80, 0–100 and 0–140 μmol/L, respectively） and the sensitivity increased（7.7, 15.5 and 17.6 folds of the fluorescence enhancement and 512, 66.2 and 37.6 ppb of the detection limit） with the rising of the thiourea-unit numbers. Furthermore the sensors exhibited excellent interference immunity to multiple environmentally and biologically relevant metal ions. Pond and tap water assay showed good practicability of the sensors. The number of the bound Hg^（2＋） equaling to that of the thiourea units and the irreversible recognition process implied a new interaction way between Hg^（2＋） and the sensor.展开更多
A pseudorotaxane and its polypseudorotaxanes formed between pillar[5]arene moieties and noctylpyrazinium cations as novel fluorescent sensors for the selective detection of halogen ions were reported.A collapse of the...A pseudorotaxane and its polypseudorotaxanes formed between pillar[5]arene moieties and noctylpyrazinium cations as novel fluorescent sensors for the selective detection of halogen ions were reported.A collapse of these pillar[5]arene-based pseudorotaxanes and polypseudorotaxanes occurred upon the addition of Cl,Br,and I（tetrabutylammonium salts）,respectively,leading to their fluorescence recovery.The fluorescence enhancement of the pseudorotaxane and the polypseudorotaxanes increases in the order of I展开更多
Extensive application of nuclear energy has caused widespread environmental uranium contamination.New detection approaches without complicated sample pretreatment and precision instruments are in demand for on-site an...Extensive application of nuclear energy has caused widespread environmental uranium contamination.New detection approaches without complicated sample pretreatment and precision instruments are in demand for on-site and in-time determination of uranyl ions in environmental monitoring, especially in an emergency situation. In this work, a simple and effective fluorescent sensor(Z)-N’-hydroxy-4-(1,2,2-triphenylvinyl)benzimidamide(TPE-A) with aggregation-induced emission(AIE) character was established and studied. It could realize to detect UO_(2)2+via quenching the fluorescence of its aggregation-induced emission, with good selectivity and sensitivity. Such strategy shows a wide linear range from 5.0 × 10^(-8)mol/L to 4.5 × 10^(-7)mol/L(R^(2)= 0.9988) with exceptional sensitivity reaching 4.7 × 10^(-9)mol/L, which is far below the limit for uranium in drinking water(30 μg/L, ca. 1.1 × 10-7mol/L) stipulated by the WHO.A response time less than four minutes make it rapid for uranyl ion measurement. It was applied for detection of uranyl ion in spiked river water samples with recoveries in the range of 98.7%-104.0%, comparable to those obtained by ICP-MS. With the advantages of portable apparatus, rapid detection process and high sensitivity, TPE-A can serve as a promising fluorescent sensor for the detection of UO_(2)2+in environmental water samples.展开更多
CdSe/CdS quantum dots （QDs） functionalized by thiourea （TU） were synthesized and used as a fluorescent sensor for mercury ion detection. The TU-functionalized QDs were prepared by bonding TU via electrostatic inte...CdSe/CdS quantum dots （QDs） functionalized by thiourea （TU） were synthesized and used as a fluorescent sensor for mercury ion detection. The TU-functionalized QDs were prepared by bonding TU via electrostatic interaction to the core/shell CdSe/CdS QDs after capping with thioglycolic acid （TGA）. It was observed that the fluorescence of the functionalized QDs was quenched upon the addition of Hg^2＋. The quantitative detection of Hg^2＋ with this fluorescent sensor could be conducted based on the linear relationship between the extent of quenching and the concentration of Hg^2＋ added in the range of 1-300 μg.L^-1, A detection limit of 0.56 μg.L^-1 was achieved. The sensor showed superior selectivity for Hg^2＋ and was successfully applied to the determination of mercury in environmental samples with satisfactory results展开更多
Glutathione(GSH)plays a critical role in maintaining oxidation-reduction homeostasis in biological systems.Considering the detection of GSH by fluorescence sensors is limited by either the short wavelength emission or...Glutathione(GSH)plays a critical role in maintaining oxidation-reduction homeostasis in biological systems.Considering the detection of GSH by fluorescence sensors is limited by either the short wavelength emission or the poor photostability,a highly stable colorimetric and ratiometric NIR fluorescent sensor(DCM-S)for GSH detection has been constructed on the basis of dicyanomethylene-4H-pyran(DCM)chromophore.The specific disulfide bond is incorporated via a carbamate linker as the GSH responsive group,which simultaneously blue-shifts and quenches the fluorescence.Upon addition of GSH,DCM-S exhibits outstanding colorimetric(from yellow to red)and ratiometric fluorescent response with the 6-fold enhancement of NIR fluorescence at 665 nm in quantum yield.More importantly,the GSH-treated DCM-S(DCM-NH_2 actually)possesses 20-fold longer fluorescence half-life period as well as much better photostability than the FDA-approved ICG.Finally,the ratiometric detection of GSH is also successfully operated in the living cell imaging,exhibiting NIR fluorescence and large Stokes shift(215 nm)with nearly no background fluorescence interference.As a consequence,DCM-S can be utilized as colorimetric and ratiometric NIR fluorescent sensor for GSH,with a great potential in the development of GSH-induced drug delivery system.展开更多
The BINOL-amino alcohol enantiomeric pair （S）-I and （R）-I are discovered to conduct both enantioselective and diastereose- lective fluorescent discrimination of the four stereoisomers of threonine derivatives. Thi...The BINOL-amino alcohol enantiomeric pair （S）-I and （R）-I are discovered to conduct both enantioselective and diastereose- lective fluorescent discrimination of the four stereoisomers of threonine derivatives. This study utilizes different fluorescence responses of one sensor at two emission wavelengths toward the stereoisomeric substrates which expands the capability of the sensor in chiral recognition. In addition, the sensor pair also allows visual recognition of the N-protected L-allo-threonine and D-allo-threonine by enantioselective precipitation.展开更多
In this paper a label-free fluorescent sensor for probing the interaction between heparin and protein was reported.Heparin,the bioactive polyanions,formed supramolecular assemblies with cationic surfactant cetyltrimet...In this paper a label-free fluorescent sensor for probing the interaction between heparin and protein was reported.Heparin,the bioactive polyanions,formed supramolecular assemblies with cationic surfactant cetyltrimethyl ammonium bromide (CTAB).The environment-dependent dye pyrene,encapsulated in hydrophobic interiors of the supramolecular assemblies worked as the fluorescence probe.Once the heparin-binding protein was added,competing interactions of protein with heparin would weaken the interaction between CTAB and heparin.As a result,the noncovalently sequestered pyrene would be released upon disassembly and the fluorescence of the released pyrene was subsequently decreased.The binding events were exemplified by protamine and Tat peptide,these processes were also verified by DLS and TEM.Such a strategy is appealing as organic synthesis was traded off against supramolecular assembly.This label-free fluorescent system is simple,selective,convenient,and can serve as a good complement to other existing methods,also this method has the potential for preprimary drug screening.展开更多
Two highly emissive pyrenoviologen derivatives were synthesized and used to fabricate fluorescent sensors for detection of picric acid(PA)with good sensitivity and selectivity.The sensitivity of the sensor was attribu...Two highly emissive pyrenoviologen derivatives were synthesized and used to fabricate fluorescent sensors for detection of picric acid(PA)with good sensitivity and selectivity.The sensitivity of the sensor was attributed to the specific electrostatic association effect of the cationic pyrenoviologens to the picrate anions,which also gave the sensor special selectivity among other compounds with similar structure.The electron transfer between them was attributed to the fluorescence response.Fluorescence lifetime measurements revealed that the quenching is static in nature.The novel and efficient pyrenoviologen derivatives-based sensors offered a strategy to fabricate real-life PA sensor.展开更多
Structurally simple salicylaldehyde-based fluoreseent sensors for amino acids have been obtained by one-step or two-step synthesis.These sensors show significant fluorescence enhancement in the presence of many amino ...Structurally simple salicylaldehyde-based fluoreseent sensors for amino acids have been obtained by one-step or two-step synthesis.These sensors show significant fluorescence enhancement in the presence of many amino acids at concentrations as low as 10~5 mol/L.The reversible reaction of the aldehydes with amino acids to form imines in aqueous solution is proposed to account for the observed fluorescence enhancement.展开更多
cAMP is an important second messenger that is capable of controlling a wide array of cellular processes, including glycogen, sugar and lipid metabolism. Here we report the design and construction of a novel geneticall...cAMP is an important second messenger that is capable of controlling a wide array of cellular processes, including glycogen, sugar and lipid metabolism. Here we report the design and construction of a novel genetically encoded fluorescent sensor for cAMP. The sensor was realized by fusing E. coli CAP protein with cpYFP, and displayed a one-fold fluorescence change towards c AMP binding. Further characterization assays demonstrated that the sensor had high affinity for cAMP and fast response kinetics.The development of our sensor could be a useful supplement to existing methods for cAMP detection.展开更多
A metalloporphyrin-based fluorescent sensor was developed to determine the acid value in frying oil.The electronic and structural performances of iron tetraphenylporphyrin(FeTPP)were theoretically investigated using t...A metalloporphyrin-based fluorescent sensor was developed to determine the acid value in frying oil.The electronic and structural performances of iron tetraphenylporphyrin(FeTPP)were theoretically investigated using time-dependent density functional theory and density functional theory at the B3LYP/LANL2DZ level.The quantified FeTPP-based fluorescent sensor results revealed its excellent performance in discriminating different analytes.In the present work,the acid value of palm olein was determined after every single frying cycle.A total of 10 frying cycles were conducted each day for 10 consecutive days.The FeTPP-based fluorescent sensor was used to quantify the acid value,and the results were compared with the chemical data obtained by conventional titration method.The synchronous fluorescence spectrum for each sample was recorded.Parallel factor analysis was used to decompose the three-dimensional spectrum data.Then,the support vector regression(SVR),partial least squares,and back-propagation artificial neural network methods were applied to build the regression models.After the comparison of the constructed models,the SVR models exhibited the highest correlation coefficients among all models,with 0.9748 and 0.9276 for the training and test sets,respectively.The findings suggested the potential of FeTPP-based fluorescent sensor in rapid monitoring of frying oil quality and perhaps also in other foods with higher oil contents.展开更多
Response speed is one of the most important evaluation criteria for CO2 sensors. In this work, we report an ultrafast CO2 fluorescent sensor based on poly[oligo（ethylene glycol） methyl ether methacrylate]-b-poly[N,N...Response speed is one of the most important evaluation criteria for CO2 sensors. In this work, we report an ultrafast CO2 fluorescent sensor based on poly[oligo（ethylene glycol） methyl ether methacrylate]-b-poly[N,N-diethylaminoethyl methacrylate-r-4-（2- methylacryloyloxyethylamino）-7-nitro-2,1,3-benzoxadiazole] [POEGMA-b-P（DEAEMA-r-NBDMA）], in which DEAEMA units act as the CO2-responsive segment and 4-nitrobenzo-2-oxa-l,3-diazole （NBD） is the chromophore. The micelles composed of this copolymer could disassemble in 2 s upon CO2 bubbling, accompanying with enhanced fluorescence emission with bathochromic shift. Furthermore, the quantum yield of the NBD chromophore increases with both the CO2 aeration time and the NBD content. Thus we attribute the fluorescent enhancement to the inhibition of the photo-induced electron transfer between unprotonated tertiary amine groups and NBD fluorophores. The sensor is durable although it is based on ＂soft＂ materials. These micellar sensors could be facilely recycled by alternative CO2/Ar purging for at least 5 times, indicating good reversibility.展开更多
A novel and simple fluorescent molecular sensor,1-pyrenecarboxaldehyde thiosemicarbazone（Hpytsc）,was synthesized.Its higher sensitivity and selectivity to mercury（Ⅱ） ion were studied through absorption and emissi...A novel and simple fluorescent molecular sensor,1-pyrenecarboxaldehyde thiosemicarbazone（Hpytsc）,was synthesized.Its higher sensitivity and selectivity to mercury（Ⅱ） ion were studied through absorption and emission channels.The UV-vis spectra show that the increasing mercury（Ⅱ） ion concentrations result in the decreasing absorption intensity.The fluorescence monomer emission of Hpytsc is enhanced upon binding mercury（Ⅱ） ion,which should be due to the 1：1 complex formation between Hpytsc and metal ion.展开更多
基金This work is supported by the National Natural Science Foundation of China(No.21804105)by the Fundamental Research Funds for the Central Universities(No.5003515037)supported by the Huazhong University of Science and Technology Start-up Fund to Xu YU.
文摘Fluorescent nanoparticles have good chemical stability and photostability,controllable optical properties and larger stokes shift.In light of their designability and functionability,the fluorescent nanoparticles are widely used as the fluorescent probes for diverse applications.To enhance the sensitivity and selectivity,the combination of the fluorescent nanoparticles with the molecularly imprinted polymer,i.e.molecularly imprinted fluorescent nanoparticles(MIFN),was an effective way.The sensor based on MIFN(the MIFN sensor)could be more compatible with the complex sample matrix,which was especially widely adopted in medical and biological analysis.In this mini-review,the construction method,detective mechanism and types of MIFN sensors are elaborated.The current applications of MIFN sensors in pharmaceutical analysis,including pesticides/herbicide,veterinary drugs/drugs residues and human related proteins,are highlighted based on the literature in the recent three years.Finally,the research prospect and development trend of the MIFN sensor are forecasted.
基金Supported by the National Natural Science Foundation of China(Nos.30672560,81172982)the Accented Project for Natural Scientific Research of Universities in Guangdong Province,China(No.05Z012)
文摘A novel fluorescent probe,6-（N,N-dimethylamino）-2-naphthoylacryl acid（ACADAN） was designed and synthesized as a fluorescent sensor for Cu^2＋ in aqueous media.Significant amplification of fluorescence signals without causing any discernible change of maximum fluorescence emission wavelength（λ max） was observed upon the addition of Cu^2＋.Importantly,ACADAN is capable of recognizing Cu^2＋ selectively in aqueous media in the presence of various biologically relevant metal ions and the prevalent toxic metal ions in the environment with high sensitivity（detection limit was 0.1 μmol/L）.
基金the financial support from the National Natural Science Foundation of China(Grant No.21978241,21878249,U1803283 and 21676218)Science Foundation of Shaanxi Province(2023-YBSF-224).
文摘The survival of human beings is inseparable from the development of agriculture.The use of pesticides is still an important means to ensure agricultural production,economic benefits and the national economy.With the abuse of pesticides,some pesticides are difficult to degrade after entering the natural world,which leads to many serious problems through the transmission and enrichment of food chains.Pesticide residue detection is a long-term challenge in the field of environment and food safety detection.In recent years,a large number of studies have been carried out on the detection of pesticide residues based on fluorescence sensing methods.This paper focuses on the application of fluorescence sensors in environmental samples or food,and analyzes the advantages and problems of existing fluorescence sensors in design,construction and data processing.Finally,the possible improvement directions in the future are discussed to promote its potential commercial value application research.
基金supported by the National Natural Science Foundation of China(No.21976211).
文摘Increasing use of silver in various fields has caused Ag^(+)pollution in water environment,taking great threats to people’s health.As a consequence,establishing rapid and reliable methods for sensitive determination of Ag^(+)is of great significance.Fluorescent(FL)sensors based on carbon dots(CDs),an excellent carbonaceous nanomaterial with strong and stable fluorescence,have absorbed extensive attentions in analysis of pollutants due to its advantages of carbon sources being readily available,low cost,easy operation and fast response.Moreover,ion-imprinting is a better way to increase the selectivity of the proposed method.Present work described an effective method for the sensitive measurement of silver ion in water samples in combination with magnetic ion-imprinted solid phase extraction and CDs based fluorescent sensor,which took full advantages of easy separation and high enrichment of magnetic solid phase extraction,high selectivity of ion-imprinting technology,and sensitivity and rapid response of fluorescent sensor from CDs.Sulfur-doped CDs derived from dithizone and magnetic ion-imprinted nanomaterial were prepared,and characterized with Fourier transform infrared spectroscopy and transmission electron microscope,etc.Magnetic Ag^(+)imprinted nanomaterial based solid phase extraction was employed for separating and enriching Ag^(+)from water samples.The significant parameters were optimized in detail.Under the optimal conditions,the proposed method provided good linearity in the range of 0.01-0.4μmol/L and low detection limit of 3 nmol/L.The reliability of the proposed method was validated with real water samples,and the results demonstrated that the proposed method was simple,robust,selective and sensitive detection tool for Ag^(+)in real water samples.
基金supported by the National Natural Science Foundation of China(Nos.22077099,22171223 and 22307102)the Innovation Capability Support Program of Shaanxi(Nos.2023-CXTD-75 and 2022KJXX-32)+5 种基金the Technology Innovation Leading Program of Shaanxi(Nos.2023KXJ-209 and 2024QCY-KXJ-142)the Key Research and Development Program of Shaanxi(No.2024GHZDXM-22)the Natural Science Basic Research Program of Shaanxi(Nos.2023-JC-YB-141 and 2022JQ-151)Young Talent Fund of Association for Science and Technology in Shaanxi,China(No.SWYY202206)the Shaanxi Fundamental Science Research Project for Chemistry&Biology(Nos.22JHZ010 and 22JHQ080)the Yan’an City Science and Technology Project(No.2022SLZDCY-002)。
文摘Benzo[4,5]imidazo[1,2-a]pyrimidine-based derivatives play crucial roles in medicines,pesticides,tracers and photoelectric materials.However,their synthesis approach still needs to be optimized,and their fluorescent properties in intracellular microenvironment are unclear.Here,a Cu(II)-catalyzed cascade coupling cyclization reaction was successfully developed to synthesize benzo[4,5]imidazo[1,2-a]pyrimidine scaffold with mild reaction conditions,broad substrate scopes and high yields.After a system study,we found that compound 4aa displayed an optimal viscosity-specific response with remarkable fluorescence enhancement(102-fold)for glycerol at 490 nm.Particularly,4aa possessed excellent structure-inherent targeting(SIT)capability for lysosome(P=0.95)with high p H stability and large Stokes shift.Importantly,4aa was validated for its effectiveness in diagnosing lysosomal storage disorders(LSD)in living cells.The 4aa also showed its potential to map the micro-viscosity and its metabolism process in zebrafish.This work not only affords an efficient protocol to fabricate benzo[4,5]imidazo[1,2-a]pyrimidine derivatives,reveals this skeleton has excellent SIT features for lysosome,but also manifests that 4aa can serve as a practical tool to monitor lysosomal viscosity and diagnose LSD.
基金supported by the National Key Research and Development Program of China(2017YFA050400 and2017YFC0906900)the National Natural Science Foundation of China(31722033,91649123,31671484,31225008,and 31470833)+4 种基金the Shanghai Science and Technology Commission(14XD1401400,16430723100,and 15YF1402600)Young Elite Scientists Sponsorship Program by China Association for Science and Technology(to YZ)Shanghai Young Top-notch Talent(to YZ)the State Key Laboratory of Bioreactor Engineering(to YY)Fundamental Research Funds for the Central Universities(to YY and YZ)
文摘The brain has very high energy requirements and consumes 20% of the oxygen and 25% of the glucose in the human body. Therefore, the molecular mechanism under- lying how the brain metabolizes substances to support neural activity is a fundamental issue for neuroscience studies. A well-known model in the brain, the astrocyte- neuron lactate shuttle, postulates that glucose uptake and glycolytic activity are enhanced in astrocytes upon neu- ronal activation and that astrocytes transport lactate into neurons to fulfill their energy requirements. Current evidence for this hypothesis has yet to reach a clear consensus, and new concepts beyond the shuttle hypothesis are emerging. The discrepancy is largely attributed to the lack of a critical method for real-time monitoring of metabolic dynamics at cellular resolution. Recent advances in fluorescent protein-based sensors allow the generation of a sensitive, specific, real-time readout of subcellular metabolites and fill the current technological gap. Here,we summarize the development of genetically encoded metabolite sensors and their applications in assessing cell metabolism in living cells and in vivo, and we believe that these tools will help to address the issue of elucidating neural energy metabolism.
基金supported by the National Natural Science Foundation of China (No. 21074085)the Priority Academic Program Development of Jiangsu Higher Education Institutions+1 种基金the Graduate Student Innovation Training Project of Jiangsu Province (No. KYLX_1241)the State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials
文摘Three novel rhodamine-based Hg^2＋ fluorescent sensors were designed and synthesized. The sensors could work in semi-aqueous solutions with nearly neutral p H and showed high selectivity and sensitivity to Hg^2＋ with remarkable fluorescence enhancement. For these three sensors, the linear working range broadened（0–80, 0–100 and 0–140 μmol/L, respectively） and the sensitivity increased（7.7, 15.5 and 17.6 folds of the fluorescence enhancement and 512, 66.2 and 37.6 ppb of the detection limit） with the rising of the thiourea-unit numbers. Furthermore the sensors exhibited excellent interference immunity to multiple environmentally and biologically relevant metal ions. Pond and tap water assay showed good practicability of the sensors. The number of the bound Hg^（2＋） equaling to that of the thiourea units and the irreversible recognition process implied a new interaction way between Hg^（2＋） and the sensor.
基金the financial support of the National Natural Science Foundation of China (No. 21202083)Natural Science Foundation of Jiangsu (Nos. BK2011055, BK2011551)the China Postdoctoral Science Foundation (No. 2012M511717)
文摘A pseudorotaxane and its polypseudorotaxanes formed between pillar[5]arene moieties and noctylpyrazinium cations as novel fluorescent sensors for the selective detection of halogen ions were reported.A collapse of these pillar[5]arene-based pseudorotaxanes and polypseudorotaxanes occurred upon the addition of Cl,Br,and I（tetrabutylammonium salts）,respectively,leading to their fluorescence recovery.The fluorescence enhancement of the pseudorotaxane and the polypseudorotaxanes increases in the order of I
基金the financial support received from the National Natural Science Foundation of China (No. 21702193)China Academy of Engineering Physics (Nos. TP03201601, TP02201711 and JMJJ20190101)。
文摘Extensive application of nuclear energy has caused widespread environmental uranium contamination.New detection approaches without complicated sample pretreatment and precision instruments are in demand for on-site and in-time determination of uranyl ions in environmental monitoring, especially in an emergency situation. In this work, a simple and effective fluorescent sensor(Z)-N’-hydroxy-4-(1,2,2-triphenylvinyl)benzimidamide(TPE-A) with aggregation-induced emission(AIE) character was established and studied. It could realize to detect UO_(2)2+via quenching the fluorescence of its aggregation-induced emission, with good selectivity and sensitivity. Such strategy shows a wide linear range from 5.0 × 10^(-8)mol/L to 4.5 × 10^(-7)mol/L(R^(2)= 0.9988) with exceptional sensitivity reaching 4.7 × 10^(-9)mol/L, which is far below the limit for uranium in drinking water(30 μg/L, ca. 1.1 × 10-7mol/L) stipulated by the WHO.A response time less than four minutes make it rapid for uranyl ion measurement. It was applied for detection of uranyl ion in spiked river water samples with recoveries in the range of 98.7%-104.0%, comparable to those obtained by ICP-MS. With the advantages of portable apparatus, rapid detection process and high sensitivity, TPE-A can serve as a promising fluorescent sensor for the detection of UO_(2)2+in environmental water samples.
基金the financial support from the National Natural Science Foundation of China (Nos. 20345006 and 20575043)
文摘CdSe/CdS quantum dots （QDs） functionalized by thiourea （TU） were synthesized and used as a fluorescent sensor for mercury ion detection. The TU-functionalized QDs were prepared by bonding TU via electrostatic interaction to the core/shell CdSe/CdS QDs after capping with thioglycolic acid （TGA）. It was observed that the fluorescence of the functionalized QDs was quenched upon the addition of Hg^2＋. The quantitative detection of Hg^2＋ with this fluorescent sensor could be conducted based on the linear relationship between the extent of quenching and the concentration of Hg^2＋ added in the range of 1-300 μg.L^-1, A detection limit of 0.56 μg.L^-1 was achieved. The sensor showed superior selectivity for Hg^2＋ and was successfully applied to the determination of mercury in environmental samples with satisfactory results
基金the National Basic Research Program(2013CB733700)the National Natural Science Foundation of China for Creative Research Groups(21421004)+2 种基金Distinguished Young Scholars(21325625)the Oriental Scholarship,the Fundamental Research Funds for the Central Universities(WJ1416005)Science and Technology Commission of Shanghai Municipality(15XD1501400)
文摘Glutathione(GSH)plays a critical role in maintaining oxidation-reduction homeostasis in biological systems.Considering the detection of GSH by fluorescence sensors is limited by either the short wavelength emission or the poor photostability,a highly stable colorimetric and ratiometric NIR fluorescent sensor(DCM-S)for GSH detection has been constructed on the basis of dicyanomethylene-4H-pyran(DCM)chromophore.The specific disulfide bond is incorporated via a carbamate linker as the GSH responsive group,which simultaneously blue-shifts and quenches the fluorescence.Upon addition of GSH,DCM-S exhibits outstanding colorimetric(from yellow to red)and ratiometric fluorescent response with the 6-fold enhancement of NIR fluorescence at 665 nm in quantum yield.More importantly,the GSH-treated DCM-S(DCM-NH_2 actually)possesses 20-fold longer fluorescence half-life period as well as much better photostability than the FDA-approved ICG.Finally,the ratiometric detection of GSH is also successfully operated in the living cell imaging,exhibiting NIR fluorescence and large Stokes shift(215 nm)with nearly no background fluorescence interference.As a consequence,DCM-S can be utilized as colorimetric and ratiometric NIR fluorescent sensor for GSH,with a great potential in the development of GSH-induced drug delivery system.
文摘The BINOL-amino alcohol enantiomeric pair （S）-I and （R）-I are discovered to conduct both enantioselective and diastereose- lective fluorescent discrimination of the four stereoisomers of threonine derivatives. This study utilizes different fluorescence responses of one sensor at two emission wavelengths toward the stereoisomeric substrates which expands the capability of the sensor in chiral recognition. In addition, the sensor pair also allows visual recognition of the N-protected L-allo-threonine and D-allo-threonine by enantioselective precipitation.
基金Financial support from the Natural Science Foundation of China (Nos.50830106,21174126),the China National Funds for Distinguished Young Scientists (No.51025312) and the Open Project of State Key Laboratory of Supramolecular Structure and Materials (No.sklssm201316) is gratefully acknowledged.
文摘In this paper a label-free fluorescent sensor for probing the interaction between heparin and protein was reported.Heparin,the bioactive polyanions,formed supramolecular assemblies with cationic surfactant cetyltrimethyl ammonium bromide (CTAB).The environment-dependent dye pyrene,encapsulated in hydrophobic interiors of the supramolecular assemblies worked as the fluorescence probe.Once the heparin-binding protein was added,competing interactions of protein with heparin would weaken the interaction between CTAB and heparin.As a result,the noncovalently sequestered pyrene would be released upon disassembly and the fluorescence of the released pyrene was subsequently decreased.The binding events were exemplified by protamine and Tat peptide,these processes were also verified by DLS and TEM.Such a strategy is appealing as organic synthesis was traded off against supramolecular assembly.This label-free fluorescent system is simple,selective,convenient,and can serve as a good complement to other existing methods,also this method has the potential for preprimary drug screening.
基金supported by the National Natural Science Foundation of China(Nos.21603016,21704081,51603016 and21704005)Shaanxi College Students Innovation and Entrepreneurship Training Program(No.S201910710282)
文摘Two highly emissive pyrenoviologen derivatives were synthesized and used to fabricate fluorescent sensors for detection of picric acid(PA)with good sensitivity and selectivity.The sensitivity of the sensor was attributed to the specific electrostatic association effect of the cationic pyrenoviologens to the picrate anions,which also gave the sensor special selectivity among other compounds with similar structure.The electron transfer between them was attributed to the fluorescence response.Fluorescence lifetime measurements revealed that the quenching is static in nature.The novel and efficient pyrenoviologen derivatives-based sensors offered a strategy to fabricate real-life PA sensor.
基金the National Natural Science Foundation of China(No.20876090)the Foundation for Research Initiation of Shaanxi University of Science&Technology(No.BJ09-15)Foundation for Innovative Research Team of Shaanxi University of Science&Technology(No.TD09-04)for financial support
文摘Structurally simple salicylaldehyde-based fluoreseent sensors for amino acids have been obtained by one-step or two-step synthesis.These sensors show significant fluorescence enhancement in the presence of many amino acids at concentrations as low as 10~5 mol/L.The reversible reaction of the aldehydes with amino acids to form imines in aqueous solution is proposed to account for the observed fluorescence enhancement.
基金National Basic Research Foundation of China(Grant No.2017YFA0505202)the National Natural Science Foundation of China(Grant No.91853107)
文摘cAMP is an important second messenger that is capable of controlling a wide array of cellular processes, including glycogen, sugar and lipid metabolism. Here we report the design and construction of a novel genetically encoded fluorescent sensor for cAMP. The sensor was realized by fusing E. coli CAP protein with cpYFP, and displayed a one-fold fluorescence change towards c AMP binding. Further characterization assays demonstrated that the sensor had high affinity for cAMP and fast response kinetics.The development of our sensor could be a useful supplement to existing methods for cAMP detection.
基金sponsored by the National Natural Science Foundation of China(No.31701685)Educational Commission of Anhui Province(KJ2021A1071)Chuzhou Municipal Science and Technology(Nos.2021GJ011,2021ZD017),China.
文摘A metalloporphyrin-based fluorescent sensor was developed to determine the acid value in frying oil.The electronic and structural performances of iron tetraphenylporphyrin(FeTPP)were theoretically investigated using time-dependent density functional theory and density functional theory at the B3LYP/LANL2DZ level.The quantified FeTPP-based fluorescent sensor results revealed its excellent performance in discriminating different analytes.In the present work,the acid value of palm olein was determined after every single frying cycle.A total of 10 frying cycles were conducted each day for 10 consecutive days.The FeTPP-based fluorescent sensor was used to quantify the acid value,and the results were compared with the chemical data obtained by conventional titration method.The synchronous fluorescence spectrum for each sample was recorded.Parallel factor analysis was used to decompose the three-dimensional spectrum data.Then,the support vector regression(SVR),partial least squares,and back-propagation artificial neural network methods were applied to build the regression models.After the comparison of the constructed models,the SVR models exhibited the highest correlation coefficients among all models,with 0.9748 and 0.9276 for the training and test sets,respectively.The findings suggested the potential of FeTPP-based fluorescent sensor in rapid monitoring of frying oil quality and perhaps also in other foods with higher oil contents.
基金financially supported by the National Natural Science Foundation of China (Nos. 51573086 and 21374058)the Opening Project of State Key Laboratory of Polymer Materials Engineering (Sichuan University) (No. sklpme2014-4-26)
文摘Response speed is one of the most important evaluation criteria for CO2 sensors. In this work, we report an ultrafast CO2 fluorescent sensor based on poly[oligo（ethylene glycol） methyl ether methacrylate]-b-poly[N,N-diethylaminoethyl methacrylate-r-4-（2- methylacryloyloxyethylamino）-7-nitro-2,1,3-benzoxadiazole] [POEGMA-b-P（DEAEMA-r-NBDMA）], in which DEAEMA units act as the CO2-responsive segment and 4-nitrobenzo-2-oxa-l,3-diazole （NBD） is the chromophore. The micelles composed of this copolymer could disassemble in 2 s upon CO2 bubbling, accompanying with enhanced fluorescence emission with bathochromic shift. Furthermore, the quantum yield of the NBD chromophore increases with both the CO2 aeration time and the NBD content. Thus we attribute the fluorescent enhancement to the inhibition of the photo-induced electron transfer between unprotonated tertiary amine groups and NBD fluorophores. The sensor is durable although it is based on ＂soft＂ materials. These micellar sensors could be facilely recycled by alternative CO2/Ar purging for at least 5 times, indicating good reversibility.
基金financed by the Graduate Innovation Foundation of Logistic Engineering University(Chongqing, China)
文摘A novel and simple fluorescent molecular sensor,1-pyrenecarboxaldehyde thiosemicarbazone（Hpytsc）,was synthesized.Its higher sensitivity and selectivity to mercury（Ⅱ） ion were studied through absorption and emission channels.The UV-vis spectra show that the increasing mercury（Ⅱ） ion concentrations result in the decreasing absorption intensity.The fluorescence monomer emission of Hpytsc is enhanced upon binding mercury（Ⅱ） ion,which should be due to the 1：1 complex formation between Hpytsc and metal ion.
