Sijunzi Decoction,as one of the classic Chinese traditional prescriptions,has been used clinically by major physicians since the Song Dynasty.This article reviewed and sorted out the literature on the effective chemis...Sijunzi Decoction,as one of the classic Chinese traditional prescriptions,has been used clinically by major physicians since the Song Dynasty.This article reviewed and sorted out the literature on the effective chemistry of Sijunzi Decoction and the mechanism of its prevention and treatment of digestive system diseases.At present,its effective chemical components are derived from the saponins and flavonoids in ginseng and licorice,and are effective for gastrointestinal mucosal injury diseases and malignant digestive system.Digestive system diseases such as tumors,functional gastrointestinal diseases,non-alcoholic fatty liver,acute liver injury,and liver failure show a multi-path,multi-target effect mechanism.This article reviews the effective chemical components and research of Sijunzi Decoction and the related mechanisms of prevention and treatment of digestive system diseases,and provides valuable clues for the follow-up research of Sijunzi Decoction.展开更多
为了研究猪圆环病毒4型(porcine circovirus type 4,PCV4)Cap蛋白功能,本研究构建了其原核表达质粒pCzn1-Cap,并对其进行了诱导表达和蛋白纯化。以纯化的重组蛋白His-Cap作为免疫原免疫BALB/c小鼠,并将SP2/0骨髓瘤细胞与免疫后的小鼠脾...为了研究猪圆环病毒4型(porcine circovirus type 4,PCV4)Cap蛋白功能,本研究构建了其原核表达质粒pCzn1-Cap,并对其进行了诱导表达和蛋白纯化。以纯化的重组蛋白His-Cap作为免疫原免疫BALB/c小鼠,并将SP2/0骨髓瘤细胞与免疫后的小鼠脾细胞融合,通过ELISA筛选得到了1株能够稳定、可持续性分泌与重组蛋白His-Cap有较高特异性反应的单克隆抗体的杂交瘤细胞株,命名为3E6G3。经测定,单克隆抗体3E6G3的抗血清效价可达1∶512000。PCV4 Cap蛋白单克隆抗体的制备将为该蛋白功能的研究和PCV4感染的快速诊断及防控奠定基础。展开更多
文摘Sijunzi Decoction,as one of the classic Chinese traditional prescriptions,has been used clinically by major physicians since the Song Dynasty.This article reviewed and sorted out the literature on the effective chemistry of Sijunzi Decoction and the mechanism of its prevention and treatment of digestive system diseases.At present,its effective chemical components are derived from the saponins and flavonoids in ginseng and licorice,and are effective for gastrointestinal mucosal injury diseases and malignant digestive system.Digestive system diseases such as tumors,functional gastrointestinal diseases,non-alcoholic fatty liver,acute liver injury,and liver failure show a multi-path,multi-target effect mechanism.This article reviews the effective chemical components and research of Sijunzi Decoction and the related mechanisms of prevention and treatment of digestive system diseases,and provides valuable clues for the follow-up research of Sijunzi Decoction.
文摘为了研究猪圆环病毒4型(porcine circovirus type 4,PCV4)Cap蛋白功能,本研究构建了其原核表达质粒pCzn1-Cap,并对其进行了诱导表达和蛋白纯化。以纯化的重组蛋白His-Cap作为免疫原免疫BALB/c小鼠,并将SP2/0骨髓瘤细胞与免疫后的小鼠脾细胞融合,通过ELISA筛选得到了1株能够稳定、可持续性分泌与重组蛋白His-Cap有较高特异性反应的单克隆抗体的杂交瘤细胞株,命名为3E6G3。经测定,单克隆抗体3E6G3的抗血清效价可达1∶512000。PCV4 Cap蛋白单克隆抗体的制备将为该蛋白功能的研究和PCV4感染的快速诊断及防控奠定基础。
