Taihu Lake is one of the five biggest lakes in China. Surface water samples from 26 sampling sites of Taihu Lake were collected. Furthermore wet chemical analysis (CODCr and BOD5) and measurement of three dimensiona...Taihu Lake is one of the five biggest lakes in China. Surface water samples from 26 sampling sites of Taihu Lake were collected. Furthermore wet chemical analysis (CODCr and BOD5) and measurement of three dimensional excitation-emission matrix (3DEEM) spectra in the laboratory have been conducted. Using parallel factor analysis (PARAFAC) model, three components of colored dissolved organic matter (CDOM) have been identified successfully, based on the analysis of 3DEEM data. The characteristics of the three components also have been described by comparing them to some components of CDOM, identified in earlier researches. Meanwhile, spatial variations of concentration for the three components in Taihu Lake have been analyzed, and the result indicates that the concentration of component 1 depends more on the situation of wastewater pollution and can be used as the indicator of wastewater pollution. The relationship between the concentrations of the three components and results of the wet chemical analysis show that none of the three components can be used as indicators of gross organic matter in water. However, the concentrations of all the three components have obvious linear relationships with the BOD5 value, especially for component 1 (r = 0.72878). Finally, the potential applications of the composition analysis based on 3DEEM and PARAFAC model in water quality monitoring have been illuminated.展开更多
The cDNA library of an ovary from Small Tail Han sheep before estrus was constructed by switching mechanism at 5' end of RNA transcript (SMART) approach. This library had a plaque titer of 1 x 109 pfu mL-1 and a 96...The cDNA library of an ovary from Small Tail Han sheep before estrus was constructed by switching mechanism at 5' end of RNA transcript (SMART) approach. This library had a plaque titer of 1 x 109 pfu mL-1 and a 96% recombinant ratio of which the fragment length of inserted average cDNA sequences was 1.0 kb. Based on bioinformatics analysis of the sequences, we obtained 338 expressed sequence tags (ESTs) from 380 cDNA clones which indicated 191 contigs. These contigs consist of 89 unmatched ESTs, 9 homologous known genes in sheep, and 93 homologous sequences in species of mouse, bovine, and human beings, including 19 sequences expressed in the ovary or follicle and 14 unknown sequences. Several candidate genes associated with sheep reproduction trait such as epidermal growth factor (EGF), estrogen receptor (ESR), Inhibin, follicle stimulating hormone receptor (FSHR), prostaglandin (PG), and transforming growth factor-β (TGF-β) were identified and the homologous were cloned from this library, which will contribute to compile expression profiles and find the major genes of prolificacy of Small Tail Han sheep.展开更多
Mg-xLi-Al alloys with Mn addition from 0.2% to 1.5 % by wt. were produced and studied. The density of the alloys is very low, between 1.21 g/cm3 and 1.64 g/cm3, while the microstructures change from single α-, (α+β...Mg-xLi-Al alloys with Mn addition from 0.2% to 1.5 % by wt. were produced and studied. The density of the alloys is very low, between 1.21 g/cm3 and 1.64 g/cm3, while the microstructures change from single α-, (α+β)-, to single β-phase with lithium content rising from 5 % to 22 % by wt. The main alloy studied was LA92 alloy with Mn addition. The results of the tensile tests show that the strength decreases with increasing lithium content, while the elongation increases sharply, and the UTS and YS rise by 26.8% and 22.7% respectively, when 0.5 % by wt. Mn is added. It is also known, by microstructure observation, SEM with EDS and X-ray analysis, that adding Mn can produce some new hard phases in the alloy, which may worsen the tensile properties.展开更多
基金Project supported by the Knowledge Innovation Project of ChineseAcademy of Sciences (No. KGCX2-SW-111).
文摘Taihu Lake is one of the five biggest lakes in China. Surface water samples from 26 sampling sites of Taihu Lake were collected. Furthermore wet chemical analysis (CODCr and BOD5) and measurement of three dimensional excitation-emission matrix (3DEEM) spectra in the laboratory have been conducted. Using parallel factor analysis (PARAFAC) model, three components of colored dissolved organic matter (CDOM) have been identified successfully, based on the analysis of 3DEEM data. The characteristics of the three components also have been described by comparing them to some components of CDOM, identified in earlier researches. Meanwhile, spatial variations of concentration for the three components in Taihu Lake have been analyzed, and the result indicates that the concentration of component 1 depends more on the situation of wastewater pollution and can be used as the indicator of wastewater pollution. The relationship between the concentrations of the three components and results of the wet chemical analysis show that none of the three components can be used as indicators of gross organic matter in water. However, the concentrations of all the three components have obvious linear relationships with the BOD5 value, especially for component 1 (r = 0.72878). Finally, the potential applications of the composition analysis based on 3DEEM and PARAFAC model in water quality monitoring have been illuminated.
文摘The cDNA library of an ovary from Small Tail Han sheep before estrus was constructed by switching mechanism at 5' end of RNA transcript (SMART) approach. This library had a plaque titer of 1 x 109 pfu mL-1 and a 96% recombinant ratio of which the fragment length of inserted average cDNA sequences was 1.0 kb. Based on bioinformatics analysis of the sequences, we obtained 338 expressed sequence tags (ESTs) from 380 cDNA clones which indicated 191 contigs. These contigs consist of 89 unmatched ESTs, 9 homologous known genes in sheep, and 93 homologous sequences in species of mouse, bovine, and human beings, including 19 sequences expressed in the ovary or follicle and 14 unknown sequences. Several candidate genes associated with sheep reproduction trait such as epidermal growth factor (EGF), estrogen receptor (ESR), Inhibin, follicle stimulating hormone receptor (FSHR), prostaglandin (PG), and transforming growth factor-β (TGF-β) were identified and the homologous were cloned from this library, which will contribute to compile expression profiles and find the major genes of prolificacy of Small Tail Han sheep.
文摘Mg-xLi-Al alloys with Mn addition from 0.2% to 1.5 % by wt. were produced and studied. The density of the alloys is very low, between 1.21 g/cm3 and 1.64 g/cm3, while the microstructures change from single α-, (α+β)-, to single β-phase with lithium content rising from 5 % to 22 % by wt. The main alloy studied was LA92 alloy with Mn addition. The results of the tensile tests show that the strength decreases with increasing lithium content, while the elongation increases sharply, and the UTS and YS rise by 26.8% and 22.7% respectively, when 0.5 % by wt. Mn is added. It is also known, by microstructure observation, SEM with EDS and X-ray analysis, that adding Mn can produce some new hard phases in the alloy, which may worsen the tensile properties.