Cold stress adversely affects rice growth,particularly at the early vegetative growth stage.In higher plants,nitrogen metabolism plays a central role in amino acid metabolism,plant defense mechanisms and productivity....Cold stress adversely affects rice growth,particularly at the early vegetative growth stage.In higher plants,nitrogen metabolism plays a central role in amino acid metabolism,plant defense mechanisms and productivity.This report investigated the effects of cold stress and supplementalγ-aminobutyric acid(GABA)under cold stress on nitrogen metabolism in rice seedlings.Cold stress resulted in a greater increase in the transformation to NH_(4)^(+)by nitrate reductase(NR)in roots,it further resulted in lower levels of NO_(3)^(-)content in roots,weakened glutamine glutamate(GOGAT/GS)pathway and elevated glutamate dehydrogenase(GDH)pathway of rice seedlings.Whereas,compared with cold stress,supplementation of GABA(2.5 mmol·L^(-1))could increase relative water content(79.43%)and biomass(34.15%)of rice seedlings.GABA could act as an amplifier of stress signal conduction/transduction to increase NR activity and promote NO_(3)^(-)assimilation in leaves.In addition,GABA elicited the Ca^(2+)signaling pathway which could promote the GDH pathway and GABA shunt,increase the activities of GS and GDH,and the expression of OsGAD2 and OsGDH family.The GABA might increase the ratio of the Glu family and avoid NH4+toxicity in order to raise the concentration of organic compounds and alleviate the harmful consequences of cold stress.Based on these observations,this study proposed that GABA mediated cold tolerance in rice seedlings by activating Ca^(2+)burst and subsequent crosstalk among Ca^(2+)signaling,GDH pathway and GABA shunt.展开更多
The Fifth Plenary Session of the 19th Central Committee of the Communist Party of China(CPC)in 2020 called for more notable and substantial progress toward promoting common prosperity for everyone by 2035,when sociali...The Fifth Plenary Session of the 19th Central Committee of the Communist Party of China(CPC)in 2020 called for more notable and substantial progress toward promoting common prosperity for everyone by 2035,when socialist modernization is achieved.This call set clear and specific tasks for China’s modernization and envisaged ways for China to achieve this goal in the next 15 years.These tasks will boost the Chinese people’s morale and mobilize greater efforts.In this new development stage,it is important for China to gain a thorough understanding of common prosperity at the conceptual,theoretical,and practical levels.展开更多
基金Supported by the National Natural Science Foundation of China(32301935)the Heilongjiang Province Natural Science Foundation Project(LH2020C005)+1 种基金the Postdoctoral Fund to Research Start-up of Heilongjiang Province(LBH-Q21077)Heilongjiang Province Applied Technology Research and Development Plan Project(GA20B101)。
文摘Cold stress adversely affects rice growth,particularly at the early vegetative growth stage.In higher plants,nitrogen metabolism plays a central role in amino acid metabolism,plant defense mechanisms and productivity.This report investigated the effects of cold stress and supplementalγ-aminobutyric acid(GABA)under cold stress on nitrogen metabolism in rice seedlings.Cold stress resulted in a greater increase in the transformation to NH_(4)^(+)by nitrate reductase(NR)in roots,it further resulted in lower levels of NO_(3)^(-)content in roots,weakened glutamine glutamate(GOGAT/GS)pathway and elevated glutamate dehydrogenase(GDH)pathway of rice seedlings.Whereas,compared with cold stress,supplementation of GABA(2.5 mmol·L^(-1))could increase relative water content(79.43%)and biomass(34.15%)of rice seedlings.GABA could act as an amplifier of stress signal conduction/transduction to increase NR activity and promote NO_(3)^(-)assimilation in leaves.In addition,GABA elicited the Ca^(2+)signaling pathway which could promote the GDH pathway and GABA shunt,increase the activities of GS and GDH,and the expression of OsGAD2 and OsGDH family.The GABA might increase the ratio of the Glu family and avoid NH4+toxicity in order to raise the concentration of organic compounds and alleviate the harmful consequences of cold stress.Based on these observations,this study proposed that GABA mediated cold tolerance in rice seedlings by activating Ca^(2+)burst and subsequent crosstalk among Ca^(2+)signaling,GDH pathway and GABA shunt.
文摘The Fifth Plenary Session of the 19th Central Committee of the Communist Party of China(CPC)in 2020 called for more notable and substantial progress toward promoting common prosperity for everyone by 2035,when socialist modernization is achieved.This call set clear and specific tasks for China’s modernization and envisaged ways for China to achieve this goal in the next 15 years.These tasks will boost the Chinese people’s morale and mobilize greater efforts.In this new development stage,it is important for China to gain a thorough understanding of common prosperity at the conceptual,theoretical,and practical levels.
