Reconstruction of osteochondral(OC)defects represents an immense challenge due to the need for synchronous regeneration of special stratified tissues.The revolutionary innovation of bioprinting provides a robust metho...Reconstruction of osteochondral(OC)defects represents an immense challenge due to the need for synchronous regeneration of special stratified tissues.The revolutionary innovation of bioprinting provides a robust method for precise fabrication of tissue-engineered OCs with hierarchical structure;however,their spatial living cues for simultaneous fulfilment of osteogenesis and chondrogenesis to reconstruct the cartilage-bone interface of OC are underappreciated.Here,inspired by natural OC bilayer features,anisotropic bicellular living hydrogels(ABLHs)simultaneously embedding articular cartilage progenitor cells(ACPCs)and bone mesenchymal stem cells(BMSCs)in stratified layers were precisely fabricated via two-channel extrusion bioprinting.The optimum formulation of the 7%GelMA/3%AlgMA hydrogel bioink was demonstrated,with excellent printability at room temperature and maintained high cell viability.Moreover,the chondrogenic ability of ACPCs and the osteogenic ability of BMSCs were demonstrated in vitro,confirming the inherent differential spatial regulation of ABLHs.展开更多
CARM1(coactivator-associated arginine methyltransferase 1)is a type I protein arginine methyltransferase and a binding protein of the p160 coactivator family.1 Moreover,the research shows that the absence of CARM1 lea...CARM1(coactivator-associated arginine methyltransferase 1)is a type I protein arginine methyltransferase and a binding protein of the p160 coactivator family.1 Moreover,the research shows that the absence of CARM1 leads to impaired adipocyte differentiation?and disrupts normal differentiation of embryonic T cells.3 In addition,other studies have confirmed that CARM1 induces the expression of pluripotent genes Oct4 and Sox2 through methylation modification of histone H3,thereby damaging embryonic stem cell differentiation.4 Furthermore,it can be indicated that CARM1 plays an important role in different types of tumors through various pathways.5 Notably,it is well known that ARAF(v-raf murine sarcoma 3611 viral oncogene homolog)regulates cell proliferation and differentiation abilities.In this study,it is revealed that CARM1 affects the epigenetic modification,transcriptome,and proteome to regulate the expression of related genes in liver cancer,thus regulating cell proliferation,cell metabolism,cell cycle,and other biological processes in liver cancer cells.These results provide a valuable theoretical basis for further exploring the cellular and molecular mechanisms of CARM1 promoting the occurrence and development of liver cancer at the cellular and molecular levels.展开更多
The pursuit of complete telomere-to-telomere(T2T)genome assembly in plants,challenged by genomic complexity,has been advanced by Oxford Nanopore Technologies(ONT),which offers ultra-long,realtime sequencing.Despite it...The pursuit of complete telomere-to-telomere(T2T)genome assembly in plants,challenged by genomic complexity,has been advanced by Oxford Nanopore Technologies(ONT),which offers ultra-long,realtime sequencing.Despite its promise,sequencing length and gap filling remain significant challenges.This study optimized DNA extraction and library preparation,achieving DNA lengths exceeding 485 kb;average N50 read lengths of 80.57 kb,reaching up to 440 kb;and maximum reads of 5.83 Mb.Importantly,we demonstrated that combining ultra-long sequencing and adaptive sampling can effectively fill gaps during assembly,evidenced by successfully filling the remaining gaps of a near-complete Arabidopsis genome assembly and resolving the sequence of an unknown telomeric region in watermelon genome.Collectively,our strategies improve the feasibility of complete T2T genomic assemblies across various plant species,enhancing genome-based research in diverse fields.展开更多
MicroRNAs(miRNAs)have been found to play an important role in human tumorigenesis.A study indicates that the plasma level of miR-933 was elevated in patients with dementia.1 Notably,miR-933(RS79402775)may contribute t...MicroRNAs(miRNAs)have been found to play an important role in human tumorigenesis.A study indicates that the plasma level of miR-933 was elevated in patients with dementia.1 Notably,miR-933(RS79402775)may contribute to the reduction of gastric cancer susceptibility.展开更多
Combining material big data with artificial intelligence constitutes the fourth paradigm of material research.However,the sluggish development of highthroughput(HT)experimentation has resulted in a lack of experimenta...Combining material big data with artificial intelligence constitutes the fourth paradigm of material research.However,the sluggish development of highthroughput(HT)experimentation has resulted in a lack of experimentally verified and validated material data,which has become the bottleneck of data-driven material research.Wet-chemical synthesis has the benefits of low equipment cost and scalability,but traditional wet-chemical techniques are time-consuming and ineffective at disclosing the interrelationships between synthesis,compositions,structures,and performance.Constructing a HT workflow in wet-chemical synthesis is crucial to achieving the preparation of multidimensional materials and establishing the composition-structure-synthesis-performance relationships of functional materials for diverse applications.In this review,the most recent development in HT wet-chemical synthesis techniques for material research are analyzed in depth.Additionally,the application of HT wet-chemical synthesis in the fabrication of advanced hydrogels and catalysts is demonstrated through illustrative instances.Finally,this review suggests possible paths for enhancing the efficiency of HT experimentation and data acquisition in order to facilitate more effective material discovery.展开更多
The maternally expressed gene 3(MEG3)acts as an antitumor component in different cancer cells.However,MEG3 variant has been shown to confer cancer susceptibility1 and certain polymorphisms within MEG3 are implicated i...The maternally expressed gene 3(MEG3)acts as an antitumor component in different cancer cells.However,MEG3 variant has been shown to confer cancer susceptibility1 and certain polymorphisms within MEG3 are implicated in cancer risk(rs7158663,rs4081134,and rs11160608).2 Moreover,MEG3-TTCC and MEG3-CCCA promote the differentiation of SCs by activating the JAK2/STAT3 signaling pathway in different degrees.3 In particular,the alleles of MEG3(AA,GA+AA)and MEG3(rs7158663)were associated with an increased risk for human liver cancer.4 Moreover,Sirt2 is a specific NAD-dependent histone deacetylase for H4–K16 and has a strong preference for histone H4K16Ac in their deacetylation activity.5 Nevertheless,the functions and mechanism of MEG3 variant on hepatocarcinogenesis have not yet been well-demonstrated.展开更多
基金This work was supported by grants from the National Key R&D Program of China(Grant Nos.2018YFA0703100 and 2022YFC2502902)the National Nature Science Foundation of China(Grant Nos.82072442,82272494,82072082,32122046,32101102)+3 种基金the Orthopaedic Medical Innovation Center of Jiangsu(CXZX202209)Key Laboratory of Orthopaedics of Suzhou(SZS2022017)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Shenzhen Fundamental Research Foundation(Grant Nos.JSGG20210629144537007,JCYJ20210324115814040,and JCYJ20210324113001005).
文摘Reconstruction of osteochondral(OC)defects represents an immense challenge due to the need for synchronous regeneration of special stratified tissues.The revolutionary innovation of bioprinting provides a robust method for precise fabrication of tissue-engineered OCs with hierarchical structure;however,their spatial living cues for simultaneous fulfilment of osteogenesis and chondrogenesis to reconstruct the cartilage-bone interface of OC are underappreciated.Here,inspired by natural OC bilayer features,anisotropic bicellular living hydrogels(ABLHs)simultaneously embedding articular cartilage progenitor cells(ACPCs)and bone mesenchymal stem cells(BMSCs)in stratified layers were precisely fabricated via two-channel extrusion bioprinting.The optimum formulation of the 7%GelMA/3%AlgMA hydrogel bioink was demonstrated,with excellent printability at room temperature and maintained high cell viability.Moreover,the chondrogenic ability of ACPCs and the osteogenic ability of BMSCs were demonstrated in vitro,confirming the inherent differential spatial regulation of ABLHs.
基金supported by the National Natural Science Foundation of China(No.82073130).
文摘CARM1(coactivator-associated arginine methyltransferase 1)is a type I protein arginine methyltransferase and a binding protein of the p160 coactivator family.1 Moreover,the research shows that the absence of CARM1 leads to impaired adipocyte differentiation?and disrupts normal differentiation of embryonic T cells.3 In addition,other studies have confirmed that CARM1 induces the expression of pluripotent genes Oct4 and Sox2 through methylation modification of histone H3,thereby damaging embryonic stem cell differentiation.4 Furthermore,it can be indicated that CARM1 plays an important role in different types of tumors through various pathways.5 Notably,it is well known that ARAF(v-raf murine sarcoma 3611 viral oncogene homolog)regulates cell proliferation and differentiation abilities.In this study,it is revealed that CARM1 affects the epigenetic modification,transcriptome,and proteome to regulate the expression of related genes in liver cancer,thus regulating cell proliferation,cell metabolism,cell cycle,and other biological processes in liver cancer cells.These results provide a valuable theoretical basis for further exploring the cellular and molecular mechanisms of CARM1 promoting the occurrence and development of liver cancer at the cellular and molecular levels.
基金supported by the Key R&D Program of Shandong Province,China(grant no.ZR202211070163)the National Natural Science Foundation of China(grant nos.32170574 and 32200249)+1 种基金the Natural Science Foundation of Shandong Province(grant nos.ZR2023QC026 and ZR2023QC106)the Young Taishan Scholars Program and Yuandu Scholars Program.
文摘The pursuit of complete telomere-to-telomere(T2T)genome assembly in plants,challenged by genomic complexity,has been advanced by Oxford Nanopore Technologies(ONT),which offers ultra-long,realtime sequencing.Despite its promise,sequencing length and gap filling remain significant challenges.This study optimized DNA extraction and library preparation,achieving DNA lengths exceeding 485 kb;average N50 read lengths of 80.57 kb,reaching up to 440 kb;and maximum reads of 5.83 Mb.Importantly,we demonstrated that combining ultra-long sequencing and adaptive sampling can effectively fill gaps during assembly,evidenced by successfully filling the remaining gaps of a near-complete Arabidopsis genome assembly and resolving the sequence of an unknown telomeric region in watermelon genome.Collectively,our strategies improve the feasibility of complete T2T genomic assemblies across various plant species,enhancing genome-based research in diverse fields.
基金supported by grants from the National Natural Science Foundation of China(No.82073130)the Science and Technology Commission of Shanghai Municipality Shanghai Science and Technology Plan Basic Research Field Project(China)(No.20JC1411400).
文摘MicroRNAs(miRNAs)have been found to play an important role in human tumorigenesis.A study indicates that the plasma level of miR-933 was elevated in patients with dementia.1 Notably,miR-933(RS79402775)may contribute to the reduction of gastric cancer susceptibility.
基金the funding support from National Key R&D Program of China(grant no.2022YFB3807700)Department of Science and Technology of Guangdong Province(no.2017ZT07Z479),Shenzhen Fundamental Research Funding(no.JCYJ20220530113802006)the Major Science,and Technology Infrastructure Project of Shenzhen Material Genome Big-Science Facilities Platform.
文摘Combining material big data with artificial intelligence constitutes the fourth paradigm of material research.However,the sluggish development of highthroughput(HT)experimentation has resulted in a lack of experimentally verified and validated material data,which has become the bottleneck of data-driven material research.Wet-chemical synthesis has the benefits of low equipment cost and scalability,but traditional wet-chemical techniques are time-consuming and ineffective at disclosing the interrelationships between synthesis,compositions,structures,and performance.Constructing a HT workflow in wet-chemical synthesis is crucial to achieving the preparation of multidimensional materials and establishing the composition-structure-synthesis-performance relationships of functional materials for diverse applications.In this review,the most recent development in HT wet-chemical synthesis techniques for material research are analyzed in depth.Additionally,the application of HT wet-chemical synthesis in the fabrication of advanced hydrogels and catalysts is demonstrated through illustrative instances.Finally,this review suggests possible paths for enhancing the efficiency of HT experimentation and data acquisition in order to facilitate more effective material discovery.
基金supported by the National Natural Science Foundation of China(No.82073130)the Science and Technology Commission of Shanghai Municipality Shanghai Science and Technology Plan Basic Research Field Project(China)(No.19JC1415200)the Science and Technology Commission of Shanghai Municipality Shanghai Science and Technology Plan Basic Research Field Project(China)(No.20JC1411400).
文摘The maternally expressed gene 3(MEG3)acts as an antitumor component in different cancer cells.However,MEG3 variant has been shown to confer cancer susceptibility1 and certain polymorphisms within MEG3 are implicated in cancer risk(rs7158663,rs4081134,and rs11160608).2 Moreover,MEG3-TTCC and MEG3-CCCA promote the differentiation of SCs by activating the JAK2/STAT3 signaling pathway in different degrees.3 In particular,the alleles of MEG3(AA,GA+AA)and MEG3(rs7158663)were associated with an increased risk for human liver cancer.4 Moreover,Sirt2 is a specific NAD-dependent histone deacetylase for H4–K16 and has a strong preference for histone H4K16Ac in their deacetylation activity.5 Nevertheless,the functions and mechanism of MEG3 variant on hepatocarcinogenesis have not yet been well-demonstrated.
