摘要
Reconstruction of osteochondral(OC)defects represents an immense challenge due to the need for synchronous regeneration of special stratified tissues.The revolutionary innovation of bioprinting provides a robust method for precise fabrication of tissue-engineered OCs with hierarchical structure;however,their spatial living cues for simultaneous fulfilment of osteogenesis and chondrogenesis to reconstruct the cartilage-bone interface of OC are underappreciated.Here,inspired by natural OC bilayer features,anisotropic bicellular living hydrogels(ABLHs)simultaneously embedding articular cartilage progenitor cells(ACPCs)and bone mesenchymal stem cells(BMSCs)in stratified layers were precisely fabricated via two-channel extrusion bioprinting.The optimum formulation of the 7%GelMA/3%AlgMA hydrogel bioink was demonstrated,with excellent printability at room temperature and maintained high cell viability.Moreover,the chondrogenic ability of ACPCs and the osteogenic ability of BMSCs were demonstrated in vitro,confirming the inherent differential spatial regulation of ABLHs.
基金
This work was supported by grants from the National Key R&D Program of China(Grant Nos.2018YFA0703100 and 2022YFC2502902)
the National Nature Science Foundation of China(Grant Nos.82072442,82272494,82072082,32122046,32101102)
the Orthopaedic Medical Innovation Center of Jiangsu(CXZX202209)
Key Laboratory of Orthopaedics of Suzhou(SZS2022017)
the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
the Shenzhen Fundamental Research Foundation(Grant Nos.JSGG20210629144537007,JCYJ20210324115814040,and JCYJ20210324113001005).
