IDEAL PLANT ARCHITECTURE1(IPA1)is a pivotal gene controlling plant architecture and grain yield.However,little is known about the effects of Triticum aestivum SQUAMOSA PROMOTER‐BINDING‐LIKE 14(TaSPL14),an IPA1 ortho...IDEAL PLANT ARCHITECTURE1(IPA1)is a pivotal gene controlling plant architecture and grain yield.However,little is known about the effects of Triticum aestivum SQUAMOSA PROMOTER‐BINDING‐LIKE 14(TaSPL14),an IPA1 ortholog in wheat,on balancing yield traits and its regulatory mechanism in wheat(T.aestivum L.).Here,we determined that the T.aestivum GRAIN WIDTH2(TaGW2)‐TaSPL14 module influences the balance between tiller number and grain weight in wheat.Overexpression of TaSPL14 resulted in a reduced tiller number and increased grain weight,whereas its knockout had the opposite effect,indicating that TaSPL14 negatively regulates tillering while positively regulating grain weight.We further identified TaGW2 as a novel interacting protein of TaSPL14 and confirmed its ability to mediate the ubiquitination and degradation of TaSPL14.Based on our genetic evidence,TaGW2 acts as a positive regulator of tiller number,in addition to its known role as a negative regulator of grain weight,which is opposite to TaSPL14.Moreover,combinations of TaSPL14‐7A and TaGW2‐6A haplotypes exhibit significantly additive effects on tiller number and grain weight in wheat breeding.Our findings provide insight into how the TaGW2‐TaSPL14 module regulates the trade‐off between tiller number and grain weight and its potential application in improving wheat yield.展开更多
Wheat culms,comprising four to six internodes,are critically involved in determining plant height and lodging resistance,essential factors for field performance and regional adaptability.This study revealed the regula...Wheat culms,comprising four to six internodes,are critically involved in determining plant height and lodging resistance,essential factors for field performance and regional adaptability.This study revealed the regulatory function of miR319 in common wheat plant height.Repression of tae-miR319 through short tandem target mimics(STTM) caused an increased plant height,while overexpression(OE) of tae-miR319 had the opposite effect.Overexpressing a miR319-resistant target gene TaPCF8(rTaPCF8),increased plant height.TaPCF8 acted as a transcription repressor of downstream genes TaIAAs,which interact physically with TaSPL14.The significant differences of indole-3-acetic acid(IAA) contents indicate the involvement of auxin pathway in miR319-mediated plant height regulation.Finally,we identified two TaPCF8 haplotypes in global wheat collections.TaPCF8-5A-Hap2,as per association and evolution examinations,was subjected to strong substantial selection throughout wheat breeding.This haplotype,associated with shorter plant height,aligns with global breeding requirements.Consequently,in high-yield wheat breeding,we proposed a potential molecular marker for marker-assisted selection(MAS).Our findings offer fresh perspectives into the molecular mechanisms that underlie the miR319–TaPCF8 module's regulation of plant height by orchestrating auxin signaling and biosynthesis in wheat.展开更多
Drought is a major environmental factor limiting wheat production worldwide,and developing drought-tolerant cultivars is a central challenge for wheat breeders globally.Therefore,it is important to identify genetic co...Drought is a major environmental factor limiting wheat production worldwide,and developing drought-tolerant cultivars is a central challenge for wheat breeders globally.Therefore,it is important to identify genetic components determining drought tolerance in wheat.In this study,we identified a wheat NAC gene(TaNAC071-A)that is tightly associated with drought tolerance by a genome-wide association study.Knockdown of TaNAC071-A in wheat attenuated plant drought tolerance,whereas its overexpression significantly enhanced drought tolerance through improved water-use efficiency and increased expression of stress-responsive genes.This heightened water-saving mechanism mitigated the yield loss caused by water deficit.Further candidate gene association analysis showed that a 108-bp insertion in the promoter of TaNAC071-A alters its expression level and contributes to variation in drought tolerance among wheat accessions.This insertion contains two MYB cis-regulatory elements(CREs)that can be directly bound by the MYB transcription activator,TaMYBL1,thereby leading to increased TaNAC071-A expression and plant drought tolerance.Importantly,introgression of this 108-bp insertion allele,TaNAC071-AIn-693,into drought-sensitive cultivars could improve their drought tolerance,demonstrating that it is a valuable genetic resource for wheat breeding.Taken together,our findings highlight a major breakthrough in determining the genetic basis underlying phenotypic variation in wheat drought tolerance and showcase the potential of exploiting CRE-containing indels for improving important agronomical traits.展开更多
基金financially supported by Beijing Natural Science Foundation (6242032)the Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-CSCB-202401)the Natural Science Foundation of Ningxia Province (2022AAC02056)
文摘IDEAL PLANT ARCHITECTURE1(IPA1)is a pivotal gene controlling plant architecture and grain yield.However,little is known about the effects of Triticum aestivum SQUAMOSA PROMOTER‐BINDING‐LIKE 14(TaSPL14),an IPA1 ortholog in wheat,on balancing yield traits and its regulatory mechanism in wheat(T.aestivum L.).Here,we determined that the T.aestivum GRAIN WIDTH2(TaGW2)‐TaSPL14 module influences the balance between tiller number and grain weight in wheat.Overexpression of TaSPL14 resulted in a reduced tiller number and increased grain weight,whereas its knockout had the opposite effect,indicating that TaSPL14 negatively regulates tillering while positively regulating grain weight.We further identified TaGW2 as a novel interacting protein of TaSPL14 and confirmed its ability to mediate the ubiquitination and degradation of TaSPL14.Based on our genetic evidence,TaGW2 acts as a positive regulator of tiller number,in addition to its known role as a negative regulator of grain weight,which is opposite to TaSPL14.Moreover,combinations of TaSPL14‐7A and TaGW2‐6A haplotypes exhibit significantly additive effects on tiller number and grain weight in wheat breeding.Our findings provide insight into how the TaGW2‐TaSPL14 module regulates the trade‐off between tiller number and grain weight and its potential application in improving wheat yield.
基金financially supported by the Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSCB-202401)the National Natural Science Foundation of China(32172045)Beijing Natural Science Foundation(6242032)。
文摘Wheat culms,comprising four to six internodes,are critically involved in determining plant height and lodging resistance,essential factors for field performance and regional adaptability.This study revealed the regulatory function of miR319 in common wheat plant height.Repression of tae-miR319 through short tandem target mimics(STTM) caused an increased plant height,while overexpression(OE) of tae-miR319 had the opposite effect.Overexpressing a miR319-resistant target gene TaPCF8(rTaPCF8),increased plant height.TaPCF8 acted as a transcription repressor of downstream genes TaIAAs,which interact physically with TaSPL14.The significant differences of indole-3-acetic acid(IAA) contents indicate the involvement of auxin pathway in miR319-mediated plant height regulation.Finally,we identified two TaPCF8 haplotypes in global wheat collections.TaPCF8-5A-Hap2,as per association and evolution examinations,was subjected to strong substantial selection throughout wheat breeding.This haplotype,associated with shorter plant height,aligns with global breeding requirements.Consequently,in high-yield wheat breeding,we proposed a potential molecular marker for marker-assisted selection(MAS).Our findings offer fresh perspectives into the molecular mechanisms that underlie the miR319–TaPCF8 module's regulation of plant height by orchestrating auxin signaling and biosynthesis in wheat.
基金supported by grants from the National Natural Science Foundation of China(31701418 and 32072002)the Natural Science Basic Research Plan in Shaanxi Province of China(2019JCW-18)and the 111 Project from the Ministry of Education of China(B07049).
文摘Drought is a major environmental factor limiting wheat production worldwide,and developing drought-tolerant cultivars is a central challenge for wheat breeders globally.Therefore,it is important to identify genetic components determining drought tolerance in wheat.In this study,we identified a wheat NAC gene(TaNAC071-A)that is tightly associated with drought tolerance by a genome-wide association study.Knockdown of TaNAC071-A in wheat attenuated plant drought tolerance,whereas its overexpression significantly enhanced drought tolerance through improved water-use efficiency and increased expression of stress-responsive genes.This heightened water-saving mechanism mitigated the yield loss caused by water deficit.Further candidate gene association analysis showed that a 108-bp insertion in the promoter of TaNAC071-A alters its expression level and contributes to variation in drought tolerance among wheat accessions.This insertion contains two MYB cis-regulatory elements(CREs)that can be directly bound by the MYB transcription activator,TaMYBL1,thereby leading to increased TaNAC071-A expression and plant drought tolerance.Importantly,introgression of this 108-bp insertion allele,TaNAC071-AIn-693,into drought-sensitive cultivars could improve their drought tolerance,demonstrating that it is a valuable genetic resource for wheat breeding.Taken together,our findings highlight a major breakthrough in determining the genetic basis underlying phenotypic variation in wheat drought tolerance and showcase the potential of exploiting CRE-containing indels for improving important agronomical traits.
