摘要
目的 探讨基于全基因组表达谱芯片筛查的BRCA1对乳腺导管内癌预后的预测价值.方法 收集广东省妇幼保健院乳腺科2014年1-6月乳腺导管内癌和乳腺浸润性导管癌的新鲜组织标本各4例,运用微阵列比较基因组杂交技术分析两者在全基因组表达谱芯片上BRCA1表达的差异,并通过免疫组化进一步检测BRCA1在70例乳腺导管内癌的表达情况,评估其对导管内癌预后的预测作用.结果 全基因组表达谱芯片结果提示:BRCA1表达在乳腺导管内癌和浸润性导管癌中存在差异.免疫组化检测到BRCA1在乳腺导管内癌的阳性率为14.3％ (10/70),其表达情况与年龄(P =0.959)、绝经状态(P=0.959)、肿瘤大小(P=0.627)、腋窝淋巴结状态(P=1.000)、HR状态(P =0.958)、HER-2状态(P=1.000)、P53表达情况(P =0.460)无明显关系.BRCA1阴性组的导管内癌伴微浸润的比例高于BRCA1阳性组(P =0.043).中位随访47个月,全组患者无病生存率为97.1％.BRCA1阴性组的无病生存率为96.7％,阳性组为100％,两组的无病生存率无明显差别(96.7％ vs 100％,P=0.569),两组的总生存率均为100％.结论 BRCA1表达可能不能预测乳腺导管内癌的预后,但乳腺导管内癌伴微浸润中BRCA1阴性比例高于乳腺导管内癌,BRCA1可能在乳腺导管内癌发生浸润过程中起作用.
Objective To investigate the role of the predictive prognostic value of BRCA1 screened by whole genome expression profiling in ductal carcinoma in situ.Methods Collected 4 cases of breast ductal carcinoma and 4 cases of breast invasive ductal carcinoma fresh samples from January 2014 to June 2014,and the difference of BRCA1 expression on whole genone expression profiling was analyzed by microarray comparative genomic hybridization.The expression of BRCA1 was detected by immunohistochemistry in 70 cases of ductal carcinoma in situ of the breast,and the prognosis of intraductal carcinoma was evaluated.Results BRCA1 gene differentially expressed in invasive ductal carcinoma and ductal carcinoma in situ by screening.The positive rate of BRCA1 protein in breast ductal carcinoma in 14.3％ (10/70),its expression had no significant relationship with age (P =0.959),menopause (P =0.959),tumor size (P =0.627),axillary lymph node status (P =1.000),HR status (P =0.958),HER-2 status (P =1.000),P53 expression (P =0.460).ductal carcinoma with micro-infiltration ratio in BRCA1 negative group was higher than BRCA1-positive group (P =0.043).The median follow-up of 47 months,Disease-free survival rate of all was 97.1％.Disease-free survival of BRCA1 negative group and BRCA1-positive group had no significant difference (96.7％ vs 100％,P =0.569),over all survival of BRCA1 negative and positive groups was 100％.Conclusions BRCA1 expression may not predict the prognosis of intraductal carcinoma,but ductal carcinoma in situ with microinvasion group ratio in BRCA1 negative was higher than ductal carcinoma in situ group,BRCA1 may take affect within ductal carcinoma infiltration process work.
出处
《国际外科学杂志》
2016年第12期-,共6页
International Journal of Surgery
基金
广东省医学科研基金(B2014023)Medical Scientific Research Foundation of Guangdong Province of China
