摘要
In one trial 360 gilthead bream fingerlings received two different feed mixtures containing two different levels of protein (diet A 400 and B 360 g?kg<sup>?1</sup>). The average initial live weight was about 2.2 ± 0.1 g fish<sup>?1</sup> in each replicate of all treatments. Sixty fish per tank were stocked randomly in 6 fiberglass tanks (1 m<sup>3</sup> each). Fish were fed with experimental diets at rate 8% of the body weight daily. The Amino Acids (AA) content was analyzed in the diets, whole fish and muscle at the end of the growth period (60 days). The results indicated that at the end of experiment gilthead bream fingerlings had reached an average live weight 18.5 and 16.2 g fish<sup>?1</sup> for fish fed diet A and B respectively. Also the increase of protein levels in the feed to 40% caused a significant increase in the content of Indispensable Amino Acid (IAA) and dispensable amino acid (as %v of whole fish and muscle of wet weight). Two methods were subsequently used to estimate the quantitative IAA requirements of gilthead bream fry based on the hypothesis that (a) the dietary requirement pattern of IAA reflected the tissue pattern and (b) the rate of daily deposition in the fish could be equated with the dietary requirement as percentage of 100 g<sup>?1</sup> diet. The quantitative IAA requirement (%) of gilthead bream (Sparus aurata) diets was as follow: Lysine 2.27, Methionine 1.09, Therionine 0.61, Leucine 2.4, Isoleucine 0.47, Histidine1.6, Arginine 1.39, Phenylalanine 1.43, Valine 1.22 and Tryptophane 0.42.
In one trial 360 gilthead bream fingerlings received two different feed mixtures containing two different levels of protein (diet A 400 and B 360 g?kg<sup>?1</sup>). The average initial live weight was about 2.2 ± 0.1 g fish<sup>?1</sup> in each replicate of all treatments. Sixty fish per tank were stocked randomly in 6 fiberglass tanks (1 m<sup>3</sup> each). Fish were fed with experimental diets at rate 8% of the body weight daily. The Amino Acids (AA) content was analyzed in the diets, whole fish and muscle at the end of the growth period (60 days). The results indicated that at the end of experiment gilthead bream fingerlings had reached an average live weight 18.5 and 16.2 g fish<sup>?1</sup> for fish fed diet A and B respectively. Also the increase of protein levels in the feed to 40% caused a significant increase in the content of Indispensable Amino Acid (IAA) and dispensable amino acid (as %v of whole fish and muscle of wet weight). Two methods were subsequently used to estimate the quantitative IAA requirements of gilthead bream fry based on the hypothesis that (a) the dietary requirement pattern of IAA reflected the tissue pattern and (b) the rate of daily deposition in the fish could be equated with the dietary requirement as percentage of 100 g<sup>?1</sup> diet. The quantitative IAA requirement (%) of gilthead bream (Sparus aurata) diets was as follow: Lysine 2.27, Methionine 1.09, Therionine 0.61, Leucine 2.4, Isoleucine 0.47, Histidine1.6, Arginine 1.39, Phenylalanine 1.43, Valine 1.22 and Tryptophane 0.42.
作者
M. M. Gaber
M. El-S. Salem
M. A. Zaki
A. M. Nour
M. M. Gaber;M. El-S. Salem;M. A. Zaki;A. M. Nour(National Institute of Oceanography and Fisheries, Cairo, Egypt;National Institute of Oceanography and Fisheries, Alexandria, Egypt;Departments of Animal and Fish Production, Faculty of Agriculture, Alexandria University, El Shatby, Egypt)
