摘要
分析植物酸性转化酶基因的保守区序列,设计一对PCR引物,以君子兰基因组DNA为模板,采用PCR方法扩增出长约500 bp的DNA片段,克隆入pGEM-T Easy载体,测序结果表明获得君子兰酸性转化酶基因家族的一个成员CMCW1,该基因片段长518 bp,不含内含子,编码172个氨基酸。其序列已在GenBank中登记(登记号为AY151269)。在GenBank中进行同源性检索的结果表明,该成员编码的氨基酸与其它植物细胞壁酸性转化酶编码的氨基酸同源性较高。
A pair of primers, designed from conserved regions of plant acid invertase genes, was used to amplify same gene by polymerase chain reaction ( PCR). As a result, one 518 bp fragment was obtained, and then cloned into pGEM -T Easy and sequenced. It has no intron and encoded 172 amino acids. This gene has been registered in GenBank with accession number AY151269. The deduced amino acid sequence is higher identical to the gene from other plant.
出处
《园艺学报》
CAS
CSCD
北大核心
2004年第2期256-258,共3页
Acta Horticulturae Sinica
基金
湖北省涝渍灾害与湿地农业重点实验室开放基金资助项目(HNKFJ2002B03)
