摘要
采用不同浓度的17β 雌二醇注射中华绒螯蟹雄性幼蟹,每隔7d注射1次,共注射5次,35d后取样。利用组织切片技术和电镜技术研究了中华绒螯蟹促雄腺显微和超微结构的变化。常规石蜡切片显示,处理组的促雄腺细胞直径明显小于对照组,同时,电镜切片结果也表明在不同浓度的处理组,促雄腺在β 雌二醇的影响下,腺细胞出现不同程度的退化,如腺细胞核变形,呈不规则的形状,核仁消失,核内异染色质增多等,而8μg/g体重的处理组效果最明显,细胞质内出现溶酶体,其它细胞器很少见。随着浓度的增加,17β 雌二醇对中华绒螯蟹促雄腺的发育及分泌抑制作用愈明显。
Different concentrations of 17β-estradiol were injected every 7 days to the immature crabs Eriocheir sinensis, which weighted about 80±5g on an average. After being raised in laboratory for 35 days, the tissue structure and ultrastructure of the androgenic gland of treated crabs were studied with tissue section and electron microscope. Paraffin section showed the androgenic gland cell secretion in treated crabs appeared degenerated and a significant decrease in the diameter of the androgenic gland cell compared with controls. Moreover, the electron microscopy showed that the nuclus was deformed and no nucleolar, while the heterochromatin was increasing in different treatment groups and lysosomes were detected in the cytoplasm in 8μg/g group.The results demonstrated 6 and 8μg/g groups had very apparent effects on androgenic gland of Eriochir sinensis H.Mile Edwards.
出处
《上海水产大学学报》
CSCD
2004年第1期10-15,共6页
Journal of Shanghai Fisheries University
基金
上海市教委重点学科重点科研项目资助(98-23)
上海市教委水产养殖重点学科基金资助。
