摘要
目的 :采用大剂量外源性糖皮质激素建立大鼠肾阳虚模型 ,研究该模型脏器重量的变化 ,并从分子水平探讨肾阳虚证病理改变及仙灵脾的调整作用和可能机制。方法 :建立肾阳虚动物模型 ,脏器称重 ,以实时荧光定量RT PCR技术 ,测定各组大鼠下丘脑 垂体 肾上腺轴钙调蛋白 (CaM )mRNA表达的变化 ,以及仙灵脾对其的影响。结果 :肾阳虚模型组大鼠各脏器重量较对照组明显减轻 ,仙灵脾灌胃有明显改善作用。肾阳虚大鼠下丘脑、肾上腺CaMmRNA水平升高 ,垂体组织CaMmRNA水平没有显著变化 ;仙灵脾能够降低下丘脑组织CaMmRNA水平 ,但对肾上腺影响不显著。结论 :下丘脑中CaMmRNA水平升高与肾阳虚证有关 ,下丘脑CaMmRNA水平的降低是仙灵脾改善肾阳虚症状的作用机制之一。
Objective:To research the action of glucocorticoid on hypothalamus pituitary adrenal cortex axis and the relationship between Epimedium and CaM in the kidney yang insufficiency model made by exogenous glucocorticoid.Methods:The kidney yang insufficiency model was made and the level of CaM mRNA was determined by real time quantitative PCR technique.Results:The weight of every viscera in model group lightened obviously compared with that in control group,TFE could adjust this tendency.CaM mRNA level increased in hypothalamus and adrenal of rats with insufficiency of the kidney yang( P<0 01,P<0 05 ),but there was no significant change in pituitary( P >0 05).CaM mRNA level of hypothalamus in rats with insufficiency of the kidney yang was reduced by Epimedium with no change in the adrenal( P >0 05).Conclusion:The up regulation of hypothalamus CaM mRNA level related to insufficiency of the kidney yang.The down regulation of hypothalamus CaM mRNA level might be one of therapeutical mechanisms by which Epimedium improved symptoms of insufficiency of the kidney yang.
出处
《滨州医学院学报》
2004年第2期87-90,共4页
Journal of Binzhou Medical University
关键词
下丘脑-垂体-肾上腺皮质轴
钙调蛋白
基因表达
仙灵脾
肾阳虚模型
hypothalamus pituitary adrenal cortex axis
calmodulin
gene expression
Epimedium
kidney yang insufficiency model
