摘要
用聚合酶链反应(PCR)法扩增两株海南省恶性疟原虫gp195的第二区,并用dd NTP链终止法测得154个氨基酸序列。实验结果表明:海南省FCC7801/HN的B3克隆及FCCM21/HN两株此区序列完全相同,与巴布亚新几内亚MAD20株很相近,但有10个氨基酸的位点有区别。
Amplification of DNA sequence of surface antigen gene,block n of gp195 from 2 Chinese isolates of Plasmodium falciparum FCC 7801/HN B3 and FCC M21/HN from Hainan,China,was established by using the polymerase chain reaction.A comparison of the 154 peptide sequences of the 2 Chinese isolates showed that they were identical,but there was a difference of 10 peptides among the established 154 peptides between the two Chinese isolates and MAD 20(a Papua New Guinea isolate).The need to determine the fragments having protective effect against malarial infection,and to study the genetic basis of the antigen polymorphism of Chinese strains of Plasmodium falciparum is evident.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1992年第2期90-94,共5页
Chinese Journal of Parasitology and Parasitic Diseases
基金
联合国开发计划署/世界银行/世界卫生组织热带病研究培训特别规划署资助
关键词
疟原虫
聚合酶链反应
脱氧核糖核酸
Plasmodium falciparum,polymerase chain reaction,DNA sequence,surface antigen.
