摘要
目的 :观察细胞外基质中纤维连接蛋白 (FN)、层粘连蛋白 (LN)在不同温度保存皮肤组织中的分布及表达 ,探讨其与皮肤冷冻保存的关系 .方法 :用FN ,LN抗体对新鲜皮肤以及 4℃ ,- 2 0℃ ,- 80℃或 - 196℃保存的皮肤作免疫组化染色 ,用图像分析仪测定并比较它们在表皮组织中的含量 ,并通过HE染色比较观察皮肤的组织结构变化 .结果 :与新鲜皮肤组相比 ,- 196℃ ,H抗冻液中皮肤组织结构保存较好 ,FN ,LN含量变化不明显 ,而 4℃ ,- 2 0℃ ,- 80℃保存皮肤的组织结构均有明显破坏 ,FN ,LN含量亦明显下降 .结论 :皮肤低温冷冻保存时细胞外基质中FN 。
AIM: To find out the distribution of fibronectin and laminin in cryopreserved skins and to investigate its relationship with cryoinjury. METHODS: Human skins were divided into 5 groups; one group was fixed immediately after harvest and the other four groups were fixed after being stored at 4℃, -20℃, -80℃ or -196℃ respectively. Fibronectin (FN) and laminin (LN) expression in them were observed with Immunohistochemical staining. RESULTS: Compared with those of the normal skin, the histological structure and FN and LN levels of skins stored at -196℃ in H anti frozen liquid did not show significant changes, while those in groups kept at 4℃, -20℃ and -80℃ changed significantly. CONCLUSION: The skin cryoinjury is associated with the loss of FN and LN in extracellular matrix.
出处
《第四军医大学学报》
北大核心
2003年第14期1268-1270,共3页
Journal of the Fourth Military Medical University
