摘要
对高效液相色谱法测定猪肉中土霉素、四环素和金霉素残留方法进行了优化,样品经0.1 mol/L Na2EDTA-Mcllvaine缓冲溶液(pH=3.5)提取,选用ZORBAX Eclipse XDB-C18色谱柱,以乙腈+0.01 mol/L NaH2PO4作为流动相梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长365 nm,进样量10μL,外标法定量。三种抗生素在0.01 mg/mL～1 mg/mL范围内线性关系良好,土霉素和四环素检出限为0.125 mg/kg,金霉素检出限为0.4 mg/kg,相对标准偏差在5%以内,三种抗生素加标回收率在72.1%～101.5%之间。该方法操作简便,能够较好地满足四环素类抗生素残留现行检测标准要求。
The method for determination of oxytetracycline,tetracycline and aureomycin in pork by high performance liquid chromatography was optimized. The sample was extracted with 0.1 mol/L Na2 EDTA-Mcllvaine buffer solution(pH=3.5),the ZORBAX Eclipse XDB-C18 column was used to separate three antibiotics,and mobile phase was gradient eluted with acetonitrile+0.01 mol/L NaH2 PO4,the flow rate was 1.0 mL/min and the column temperature was 30℃,detection wavelength was 365 nm,injection volume was 10 μL,external standard method was selected to quantitative analysis. The linearity of three antibiotics was good in the range of 0.01 mg/mL ~ 1 mg/mL,the detection limit of oxytetracycline and tetracycline was 0.125 mg/kg,the detection limit of aureomycin was 0.4 mg/kg,and relative standard deviation was within 5%,the recoveries of three antibiotics were between 72.1% and 101.5%. The method is simple and convenient,and meets the requirements of current detection standards for tetracycline antibiotic residues.
作者
范露
靖涵之
FAN Lu;JING Han-zhi(College of Food&Biology Science and Technology,Wuhan institute of design and sciences,430205)
出处
《中国食品添加剂》
CAS
2019年第3期162-168,共7页
China Food Additives
关键词
高效液相色谱法
猪肉
土霉素
四环素
金霉素
测定
high performance liquid chromatography
pork
oxytetracycline
tetracycline
aureomycin
determination
