摘要
目的:测定栀子不同部位(皮、仁、须)指标性成分含量及对其指纹图谱进行研究,比较栀子不同部位成分含量及组成差异,为栀子皮、仁药效差异提供理论依据;探究栀子须的开发利用价值,避免栀子药用资源浪费。方法:栀子苷、西红花苷Ⅰ含量测定采用HPLC法;总环烯迷萜苷含量测定采用紫外分光光度法,对栀子不同部位3个成分含量进行分析。采集栀子不同部位HPLC指纹图谱,分别建立不同产地不同加工方法栀子不同部位HPLC指纹图谱共有模式,并采用相似度评价系统软件进行数据分析;对栀子不同部位指纹图谱进行比对分析。结果:河南、福建、江西栀子皮、仁指标性成分的含量变化相同,栀子苷含量为福建>河南>江西,福建栀子皮中3种成分含量远远高于河南、江西。西红花苷Ⅰ和总环烯迷萜苷含量为福建>江西>河南,福建、江西总环烯迷萜苷含量远远高于河南。不同产地的栀子须中3个指标性成分含量比较,栀子苷、西红花苷I含量为福建>江西、河南;总环烯迷萜苷含量为福建>江西>河南。同一部位,栀子皮指纹图谱共标定22个共有峰,除了S13~S15外,其他样品的相似度在0.9以上;栀子仁指纹图谱共标定22个共有峰,除了S22~S30外,其他样品的相似度在0.9以上;栀子须指纹图谱共标定16个共有峰,除了S7~S9外,其他样品的相似度在0.9以上。不同部位,栀子须与皮、仁指纹图谱差别较大,须部位峰数减少,峰2,3,5的峰高须>皮>仁;仁部无峰X,峰高须>皮;除峰17外,仁部位图谱各峰的峰高均高于皮部位。结论:栀子皮、仁部位指标性成分含量存在显著差异;栀子须中总苷含量较高,可用于提取总环烯醚萜苷。所建指纹图谱可以反映栀子不同部位成分含量差异及种类分布,为栀子的药效物质基础研究提供理论支撑,为临床上栀皮、栀仁分开使用的科学性、合理性提供理论支持。
Objective: To determine the content of index components in different parts of Gardenia jasminoides ( pericarp,seeds,whiskers) ,study the fingerprint,and compare the contents and compositions differences of different parts of G. jasminoides,in order to provide the theoretical basis for different efficacies of G. jasminoides pericarp and seeds,explore the exploitation and utilization values of G. jasminoides whiskers,and avoid waste of gardenia medicinal resources. Method: The contents of geniposide and crocetin Ⅰ was were determined by HPLC,the content of total iridoid glycosides was determined by ultraviolet spectrophotometry,and three index components in different parts of G. jasminoides were analyzed. HPLC fingerprints of different parts of G. jasminoides were collected,the common pattern of HPLC fingerprints of different parts of G. jasminoides of different origins and with different processing methods was established,and the similarity evaluation software was used for data analysis;comparative analysis on fingerprints of different parts of G. jasminoides was conducted. Result: Content change of index components in G. jasminoides pericarp and seeds from Henan,Fujian and Jiangxi were the same. Content of geniposide: Fujian > Henan > Jiangxi, the contents of three components in G. jasminoides pericarp from Fujian were much higher than those from Henan and Jiangxi,the contents of crocetin Ⅰ and total iridoid glycosides: Fujian > Jiangxi > Henan,the contents of total iridoid glycosides from Fujian, Jiangxi were much higher than those from Henan. The order of three index components in G. jasminoides whiskers from different origins from high to low,the content of geniposide and crocetin Ⅰ was Fujian > Jiangxi and Henan, the content of total iridoid glycosides was Fujian > Jiangxi > Henan. In the same part,there were 22 common peaks in the fingerprints of G. jasminoides pericarp,except for S13-S15,the similarity of other samples were more than 0. 9;the fingerprints of G. jasminoides seeds had 22 common peaks,except for S22-S30,the similarities of other samples were more than 0. 9;the fingerprints of G. jasminoides whiskers had 16 common peaks,except for S7-S9,the similarities of other samples were more than 0. 9. In different parts,the fingerprints of G. jasminoides whiskers were significant different from those of pericarp and seeds,the number of peaks in G. jasminoides whiskers reduced,the order of height of peaks 2,3,5 of G. jasminoides from high to low were whiskers > gardenia > seeds. There was not peak X in the seeds,the height of peak X of gardenia in whiskers was higher than that in pericarp,except for the peak 17,the height of all peaks in seeds were higher than that in whiskers. Conclusion: There are significant differences in the contents of index components in G. jasminoides pericarp and seeds. The content of total glycosides in gardenia is high,suggesting that it can be used to extract total iridoid glycosides. The fingerprints can reflect the content difference and species distribution of different parts of G. jasminoides,so as to provide theoretical support for the studies for pharmacodynamic material basis of G. jasminoides and the scientificity and rationality of the separate application of G. jasminoides pericarp and seeds.
作者
梁献葵
王艳慧
雷敬卫
龚海燕
唐维维
谢彩侠
杨春静
LIANG Xian-kui;WANG Yan-hui;LEI Jing-wei;GONG Hai-yan;TANG Wei-wei;XIE Cai-xia;YANG Chun-jing(Henan University of Chinese Medicine,Zhengzhou 450046,China;Zhongjing Wanxi Pharmaceutical Co.Ltd.,Zhengzhou 450046,China;The Third Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450003,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第4期193-200,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南省中药材开发工程技术研究中心项目(142102313109)
河南省科技科技攻关项目(162102310448)
关键词
栀子皮
栀子仁
栀子须
含量测定
指纹图谱
pericarp of Gardenia jasminoides
seeds of Gardenia jasminoides
whiskers of Gardenia jasminoides
content determination
fingerprint
