摘要
目的:探究雷公藤甲素对人子宫颈微血管生成的抑制作用,并探讨其作用机制。方法:选择人子宫颈微血管内皮细胞(HCer MEC)作为研究对象,体外培养过程中分别给予0、5、10和20、40 ng/ml的雷公藤甲素处理,采用CCK-8法测定其对细胞增殖的影响,transwell实验检测细胞的迁移能力,western blotting检测细胞血管内皮生长因子(VEGF)表达的变化。结果:雷公藤甲素抑制HCer MEC的增殖活性和细胞迁移能力,并呈剂量依赖关系(P<0.05)。雷公藤甲素能够抑制HCer MEC细胞内VEGF的表达,药物浓度越高抑制作用越强。结论:雷公藤甲素可以抑制HCerMEC细胞增殖和迁移活性,该作用与其抑制VEGF表达有关。
Objective: To investigate the inhibitory effect of Triptolide on vasculogenesis of human cervical microvascular endothelial cells, and to explore the mechanism. Methods: Human cervical microvascular endothelial cells(HCerMECs) were used as research subject, and treated with different concentrations of Triptolide(0, 5, 10, 20 and 40 ng/ml) in vitro. The effect of Triptolide on cell proliferation was determined by CCK-8, the cell migration ability was detected by Transwell assay while the expression of vascular endothelial growth factor(VEGF) was examined by western blotting. Results: Triptolide inhibited the proliferation and migration of HCerMECs in a dose-dependent manner(P<0.05). In addition, Triptolide could inhibit the expression of VEGF in HCerMECs in a concentration-dependent manner. Conclusions: Triptolide could inhibit the proliferation and migration activity of HCerMECs which is related with the suppression of VEGF expression.
作者
张雅莉
刁云云
张春泽
ZHANG Yali;DIAO Yunyun;ZHANG Chunze(Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Hebei North College,Xuanhua 075100,Hebei,China;Department of Anorectal Surgery,Tianjin Peoples Hospital,Tianjin 300121,China)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2019年第2期196-199,共4页
Chinese Journal of Cancer Biotherapy
