摘要
目的了解广西藤县人群华支睾吸虫的感染状况及其分子鉴定,为当地华支睾吸虫病的防控提供理论依据。方法2016年11月,在广西藤县采用分层整群随机抽样抽取天平镇和金鸡镇各1个自然村作为调查点。收集3岁以上常驻居民的粪便,用改良加藤厚涂片法(一粪三检)检测华支睾吸虫虫卵并计数。通过问卷调查收集被调查者社会人口学等相关信息。从镜检阳性样本中随机选取部分新鲜粪样提取DNA, PCR扩增华支睾吸虫内转录间隔区2 (ITS2),测序后在NCBI核酸数据库作比对分析。结果共调查400人,检出华支睾吸虫虫卵阳性208人,感染检出率为52.0%(208/400),感染者以轻度感染为主(69.2%, 144/208)。天平镇华支睾吸虫感染检出率为66.5%(133/200),高于金鸡镇的37.5%(75/200)(χ2=33.69, P <0.01)。男性感染检出率为58.7%(131/223),高于女性的43.5%(77/177)(χ2=9.18, P <0.01); 30～39岁组感染检出率最高,为63.8%(60/94);高中及以上学历人群感染检出率最高,为68.0%(17/25)。不同年龄组、教育程度人群感染检出率差异有统计学意义(χ2=37.1, 23.02; P <0.01)。PCR扩增出156 bp的华支睾吸虫特异性条带,其序列与越南岘港分离株ITS2 (GenBank登录号:MF319655)的一致性为100%。结论广西藤县人群华支睾吸虫感染检出率为52.0%,属于超重流行区;男性、青壮年是华支睾吸虫高感染人群。广西藤县的华支睾吸虫ITS2序列与越南岘港分离株的基因型一致。
Objective To understand the infection status and identify the isolates of Clonorchis sinensis in human population in Tengxian County, Guangxi Zhuang Autonomous Region, in order to provide base information for the control of clonorchiasis. Methods Two villages from Tianping Town and Jinji Town were selected for the survey by stratified cluster random sampling method. Demographic and relative information were collected through a questionnaire survey. Fecal samples were collected from villagers with age over 3 year-old and eggs of C. sinensis in fecal samples were microscopically examined using a modified Kato-Katz technique in triplicate. The information for the distribution of C. sinensis infection in residents with different gender, age and education was analyzed. Egg-positive fecal samples were selected for DNA extraction. The fragment of internal transcribed spacer 2(ITS2) of C.sinensis was amplified using PCR for DNA sequencing. The obtained sequences were compared with those deposited in GenBank. Results A total of 400 individuals in the two villages were enrolled for investigation, of which 208 were infected with C. sinensis. The prevalence of C. sinensis was 52.0%(208/400), majority of them were mild infection(69.2%, 144/208). The prevalence in Tianping Town(66.5%, 133/200) was higher than that in Jinji Town(37.5%, 75/200) with statistical significance( χ2= 33.69, P < 0.01). The prevalence in males(58.7%, 131/223) was significantly higher than that in females(43.5%, 77/177)( χ2= 9.18, P < 0.01). The highest prevalence appeared in the 30 ~39 years group(63.8%, 60/94). Individuals who received senior high school education and above had the highest prevalence(68.0%, 17/25). The prevalence was significantly different among the age groups( χ2= 37.1, P <0.01), as it was for different levels of education( χ2= 23.02, P < 0.01). The sequence of 156 bp ITS2 fragment amplified from the DNA isolated from the fecal eggs was identical to the isolate of C. sinensis from Vietnam Da Nang(accession number: MF319655). Conclusion The overall prevalence of C. sinensis in Tengxian County was52.0%, a heavily endemic area for clonorchiasis. The infection was preferable in males with middle age and higher education. The sequence of C. sinensis ITS2 isolated from this County was identical to that of a Vietnam Da Nang isolate.
作者
徐梦
黄文
欧深
尹建海
曹胜魁
蒙丽玉
曹建平
沈玉娟
XU Meng;HUANG Wen;OU Shen;YIN Jian-hai;CAO Sheng-kui;MENG Li-yu;CAO Jian-ping;SHEN Yu-juan(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Chinese Center for Tropical Diseases Research,WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases,Ministry of Science and Technology,Key Laboratory of Parasite and Vector Biology,Ministry of Health,Shanghai 200025,China;Tengxian Center for Disease Control and Prevention,Tengxian 543300,China)
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2019年第1期28-32,共5页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家公益性卫生行业科研专项(No.201502021)
国家科技重大专项(No.2018ZX10713001)
上海市公共卫生三年行动计划(No.15GWZK0101)~~
关键词
华支睾吸虫
感染率
横断面研究
分离株
内转录间隔区2
Clonorchis sinensis
Prevalence
Cross-sectional study
Isolate
Internal transcribed spacers 2
