摘要
采用单因素法和响应曲面法优化热水提取法提取血耳总糖的工艺条件,得到最佳提取条件:液料比124∶1、提取时间120 min和提取温度100℃,其总糖提取率可达到35.347%±0.551%。利用Sevage法、冻融法、透析和DEAE-Sepharose CL-6B柱层析等方法对血耳粗多糖进行分离纯化,得到均一组分(命名为TSP-Ⅱ)。TSP-Ⅱ的回收率为31.56%,相对分子质量是350 ku,糖含量和蛋白质含量分别为97.3%和1.05%。红外光谱显示TSP-II具有糖类物质的特征吸收峰。透射电子显微镜观察TSP-II的微观结构,显示TSP-II是无分支结构的多糖,糖链之间相互缠绕。通过1μg/mL脂多糖诱导小鼠巨噬细胞RAW264.7产生炎症因子(TNF-α、IL-6和COX-2)。经脂多糖诱导的细胞添加TSP-II(75,150μg/mL)后,3种炎症因子的m RNA表达量均明显降低,说明血耳多糖TSP-II具有良好的抗炎症作用。
To obtain the optimal processing conditions for maximum total sugar yield, single factor investigation and response surface methodology(RSM) were employed. The optimal processing conditions were as follows: liquid to solid ratio 124 mL/g, extraction time 120 min, extraction temperature 100 ℃. The experimental yield was 35.347%±0.551%. A polysaccharide fraction from Tremella samguinea Peng(named TSP-II) was purified by Sevag, frozen-thawed, and DEAE-Sepharose CL-6 B column chromatography. The yield of TSP-Ⅱ was 31.56%. Its relative molecular weight was 350 ku, the content of total sugar was 97.3%, and protein was 1.05%. Characteristic peaks of polysaccharide TSP-II appeared on it fourier transform infrared spectrum(FTIR). Transmission electron microscopy(TEM) analysis revealed a primary non-branched and entangled state in its microstructure. TSP-II(75, 150 μg/mL) showed significant inhibitory activities on TNF-α, IL-6, and COX-2 gene expressions in 1 μg/mL LPS-stimulated RAW264.7 mouse macrophage cells.These results suggested the potential effects of TSP-II on anti-inflammatory properties.
作者
王昭晶
曹森
曾亚威
罗巅辉
Wang Zhaojing;Cao Sen;Zeng Yawei;Luo Dianhui(Huaqiao University,Department of Bioengineering and Biotechnology,Xiamen 361021,Fujian)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2019年第1期96-102,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(31201314)
关键词
血耳
响应曲面法
微观结构
抗炎症作用
Tremella samguinea Peng
response surface methodology(RSM)
microstructure
anti-inflammatory properties
