摘要
应用荧光原位杂交技术,以人Y染色体长臂上q12区的高度重复序列DNA探针pY3.4,分别与人和棕色田鼠的中期染色体标本及间期细胞核进行杂交,检测人和棕色田鼠的Y染色体.结果显示,人的中期染色体上和间期细胞核中发现了杂交信号,棕色田鼠的未发现杂交信号,由此讨论了棕色田鼠DNA与人Y染色体pY3.4的同源性和棕色田鼠的FISH结果,以便进一步研究棕色田鼠Y染色体。
The purpose of the investigation was to detect the Y chromosome of human and Microtus mandarinus with the satellite DNA probe in human′s Yq by fluorescence in situ hybridization(FISH). We hybridizied the probe with the metaphase chromosome and interphase nucleus specimens of humanbeing and Microtus mandarinus respectively.The results showed that there was hybridization signal on metaphase chromosome and interphase nucleus of human,but no signal on metaphase chromosome and interphase nucleus of Microtus mandarinus.According to the results,we discussed the homologue between pY3.4 in Y chromosome of humanbeing and DNA of Microtus mandarinus and FISH result of Microtus mandarinus.
出处
《遗传》
CAS
CSCD
北大核心
2003年第5期517-520,共4页
Hereditas（Beijing)
基金
国家自然科学基金资助项目(39970405
30270738)
徐州师范大学学工处基金资助
